Cultures of Dinophysis sacculus, D. acuminata and pectenotoxin 2 affect gametes and fertilization success of the Pacific oyster, Crassostrea gigas
International audience Harmful algal blooms (HABs) of toxic species of the dinoflagellate genus Dinophysis are a threat to human health as they are mainly responsible for diarrheic shellfish poisoning (DSP) in the consumers of contaminated shellfish. Such contamination leads to shellfish farm closur...
Published in: | Environmental Pollution |
---|---|
Main Authors: | , , , , , , , , , , , |
Other Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2020
|
Subjects: | |
Online Access: | https://hal.archives-ouvertes.fr/hal-02880050 https://hal.archives-ouvertes.fr/hal-02880050/document https://hal.archives-ouvertes.fr/hal-02880050/file/Gaillard%20et%20al%202020%20ENPO.pdf https://doi.org/10.1016/j.envpol.2020.114840 |
id |
ftccsdartic:oai:HAL:hal-02880050v1 |
---|---|
record_format |
openpolar |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Fertilization success Oyster gametes Pectenotoxins Okadaic acid Dinophysis spp [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography |
spellingShingle |
Fertilization success Oyster gametes Pectenotoxins Okadaic acid Dinophysis spp [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography Gaillard, Sylvain Le Goïc, Nelly Malo, Florent Boulais, Myrina Fabioux, Caroline Zaccagnini, Lucas, carpentier, liliane Sibat, Manoëlla, Réveillon, Damien Sechet, Véronique Hess, Philipp Hegaret, Helene Cultures of Dinophysis sacculus, D. acuminata and pectenotoxin 2 affect gametes and fertilization success of the Pacific oyster, Crassostrea gigas |
topic_facet |
Fertilization success Oyster gametes Pectenotoxins Okadaic acid Dinophysis spp [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography |
description |
International audience Harmful algal blooms (HABs) of toxic species of the dinoflagellate genus Dinophysis are a threat to human health as they are mainly responsible for diarrheic shellfish poisoning (DSP) in the consumers of contaminated shellfish. Such contamination leads to shellfish farm closures causing major economic and social issues. The direct effects of numerous HAB species have been demonstrated on adult bivalves, whereas the effects on critical early life stages remain relatively unexplored. The present study aimed to determine the in vitro effects of either cultivated strains of D. sacculus and D. acuminata isolated from France or their associated toxins (i.e. okadaic acid (OA) and pectenotoxin 2 (PTX2)) on the quality of the gametes of the Pacific oyster Crassostrea gigas. This was performed by assessing the ROS production and viability of the gametes using flow cytometry, and fertilization success using microscopic counts. Oocytes were more affected than spermatozoa and their mortality and ROS production increased in the presence of D. sacculus and PTX2, respectively. A decrease in fertilization success was observed at concentrations as low as 0.5 cell mL-1 of Dinophysis spp. and 5 nM of PTX2, whereas no effect of OA could be observed. The effect on fertilization success was higher when both gamete types were concomitantly exposed compared to separate exposures, suggesting a synergistic effect. Our results also suggest that the effects could be due to cell-to-cell contact. These results highlight a potential effect of Dinophysis spp. and PTX2 on reproduction and recruitment of the Pacific oyster. |
author2 |
Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) ISBlue - Interdisciplinary Graduate School for the Blue planet (2017) ANR-17-EURE-0015,ISBlue,Interdisciplinary Graduate School for the Blue planet(2017) |
format |
Article in Journal/Newspaper |
author |
Gaillard, Sylvain Le Goïc, Nelly Malo, Florent Boulais, Myrina Fabioux, Caroline Zaccagnini, Lucas, carpentier, liliane Sibat, Manoëlla, Réveillon, Damien Sechet, Véronique Hess, Philipp Hegaret, Helene |
author_facet |
Gaillard, Sylvain Le Goïc, Nelly Malo, Florent Boulais, Myrina Fabioux, Caroline Zaccagnini, Lucas, carpentier, liliane Sibat, Manoëlla, Réveillon, Damien Sechet, Véronique Hess, Philipp Hegaret, Helene |
author_sort |
Gaillard, Sylvain |
title |
Cultures of Dinophysis sacculus, D. acuminata and pectenotoxin 2 affect gametes and fertilization success of the Pacific oyster, Crassostrea gigas |
title_short |
Cultures of Dinophysis sacculus, D. acuminata and pectenotoxin 2 affect gametes and fertilization success of the Pacific oyster, Crassostrea gigas |
title_full |
Cultures of Dinophysis sacculus, D. acuminata and pectenotoxin 2 affect gametes and fertilization success of the Pacific oyster, Crassostrea gigas |
title_fullStr |
Cultures of Dinophysis sacculus, D. acuminata and pectenotoxin 2 affect gametes and fertilization success of the Pacific oyster, Crassostrea gigas |
title_full_unstemmed |
Cultures of Dinophysis sacculus, D. acuminata and pectenotoxin 2 affect gametes and fertilization success of the Pacific oyster, Crassostrea gigas |
title_sort |
cultures of dinophysis sacculus, d. acuminata and pectenotoxin 2 affect gametes and fertilization success of the pacific oyster, crassostrea gigas |
publisher |
HAL CCSD |
publishDate |
2020 |
url |
https://hal.archives-ouvertes.fr/hal-02880050 https://hal.archives-ouvertes.fr/hal-02880050/document https://hal.archives-ouvertes.fr/hal-02880050/file/Gaillard%20et%20al%202020%20ENPO.pdf https://doi.org/10.1016/j.envpol.2020.114840 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0269-7491 EISSN: 1873-6424 Environmental Pollution https://hal.archives-ouvertes.fr/hal-02880050 Environmental Pollution, Elsevier, 2020, 265, Part B, pp.114840. ⟨10.1016/j.envpol.2020.114840⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envpol.2020.114840 hal-02880050 https://hal.archives-ouvertes.fr/hal-02880050 https://hal.archives-ouvertes.fr/hal-02880050/document https://hal.archives-ouvertes.fr/hal-02880050/file/Gaillard%20et%20al%202020%20ENPO.pdf doi:10.1016/j.envpol.2020.114840 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1016/j.envpol.2020.114840 |
container_title |
Environmental Pollution |
container_volume |
265 |
container_start_page |
114840 |
_version_ |
1766394197319876608 |
spelling |
ftccsdartic:oai:HAL:hal-02880050v1 2023-05-15T15:58:27+02:00 Cultures of Dinophysis sacculus, D. acuminata and pectenotoxin 2 affect gametes and fertilization success of the Pacific oyster, Crassostrea gigas Gaillard, Sylvain Le Goïc, Nelly Malo, Florent Boulais, Myrina Fabioux, Caroline Zaccagnini, Lucas, carpentier, liliane Sibat, Manoëlla, Réveillon, Damien Sechet, Véronique Hess, Philipp Hegaret, Helene Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) ISBlue - Interdisciplinary Graduate School for the Blue planet (2017) ANR-17-EURE-0015,ISBlue,Interdisciplinary Graduate School for the Blue planet(2017) 2020 https://hal.archives-ouvertes.fr/hal-02880050 https://hal.archives-ouvertes.fr/hal-02880050/document https://hal.archives-ouvertes.fr/hal-02880050/file/Gaillard%20et%20al%202020%20ENPO.pdf https://doi.org/10.1016/j.envpol.2020.114840 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envpol.2020.114840 hal-02880050 https://hal.archives-ouvertes.fr/hal-02880050 https://hal.archives-ouvertes.fr/hal-02880050/document https://hal.archives-ouvertes.fr/hal-02880050/file/Gaillard%20et%20al%202020%20ENPO.pdf doi:10.1016/j.envpol.2020.114840 info:eu-repo/semantics/OpenAccess ISSN: 0269-7491 EISSN: 1873-6424 Environmental Pollution https://hal.archives-ouvertes.fr/hal-02880050 Environmental Pollution, Elsevier, 2020, 265, Part B, pp.114840. ⟨10.1016/j.envpol.2020.114840⟩ Fertilization success Oyster gametes Pectenotoxins Okadaic acid Dinophysis spp [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography info:eu-repo/semantics/article Journal articles 2020 ftccsdartic https://doi.org/10.1016/j.envpol.2020.114840 2021-12-19T01:03:28Z International audience Harmful algal blooms (HABs) of toxic species of the dinoflagellate genus Dinophysis are a threat to human health as they are mainly responsible for diarrheic shellfish poisoning (DSP) in the consumers of contaminated shellfish. Such contamination leads to shellfish farm closures causing major economic and social issues. The direct effects of numerous HAB species have been demonstrated on adult bivalves, whereas the effects on critical early life stages remain relatively unexplored. The present study aimed to determine the in vitro effects of either cultivated strains of D. sacculus and D. acuminata isolated from France or their associated toxins (i.e. okadaic acid (OA) and pectenotoxin 2 (PTX2)) on the quality of the gametes of the Pacific oyster Crassostrea gigas. This was performed by assessing the ROS production and viability of the gametes using flow cytometry, and fertilization success using microscopic counts. Oocytes were more affected than spermatozoa and their mortality and ROS production increased in the presence of D. sacculus and PTX2, respectively. A decrease in fertilization success was observed at concentrations as low as 0.5 cell mL-1 of Dinophysis spp. and 5 nM of PTX2, whereas no effect of OA could be observed. The effect on fertilization success was higher when both gamete types were concomitantly exposed compared to separate exposures, suggesting a synergistic effect. Our results also suggest that the effects could be due to cell-to-cell contact. These results highlight a potential effect of Dinophysis spp. and PTX2 on reproduction and recruitment of the Pacific oyster. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific Environmental Pollution 265 114840 |