Cultures of Dinophysis sacculus, D. acuminata and pectenotoxin 2 affect gametes and fertilization success of the Pacific oyster, Crassostrea gigas

• Published in: Environmental Pollution
• Main Authors: Gaillard, Sylvain, Le Goïc, Nelly, Malo, Florent, Boulais, Myrina, Fabioux, Caroline, Zaccagnini, Lucas, carpentier, liliane, Sibat, Manoëlla, Réveillon, Damien, Sechet, Véronique, Hess, Philipp, Hegaret, Helene
• Other Authors: Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), ISBlue - Interdisciplinary Graduate School for the Blue planet (2017), ANR-17-EURE-0015,ISBlue,Interdisciplinary Graduate School for the Blue planet(2017)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2020
• Subjects: Fertilization success; Oyster gametes; Pectenotoxins; Okadaic acid; Dinophysis spp; [SDE.BE]Environmental Sciences/Biodiversity and Ecology; [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology; [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology; [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography; Pacific; Crassostrea gigas; Pacific oyster
• Online Access: https://hal.archives-ouvertes.fr/hal-02880050; https://hal.archives-ouvertes.fr/hal-02880050/document; https://hal.archives-ouvertes.fr/hal-02880050/file/Gaillard%20et%20al%202020%20ENPO.pdf; https://doi.org/10.1016/j.envpol.2020.114840

International audience Harmful algal blooms (HABs) of toxic species of the dinoflagellate genus Dinophysis are a threat to human health as they are mainly responsible for diarrheic shellfish poisoning (DSP) in the consumers of contaminated shellfish. Such contamination leads to shellfish farm closures causing major economic and social issues. The direct effects of numerous HAB species have been demonstrated on adult bivalves, whereas the effects on critical early life stages remain relatively unexplored. The present study aimed to determine the in vitro effects of either cultivated strains of D. sacculus and D. acuminata isolated from France or their associated toxins (i.e. okadaic acid (OA) and pectenotoxin 2 (PTX2)) on the quality of the gametes of the Pacific oyster Crassostrea gigas. This was performed by assessing the ROS production and viability of the gametes using flow cytometry, and fertilization success using microscopic counts. Oocytes were more affected than spermatozoa and their mortality and ROS production increased in the presence of D. sacculus and PTX2, respectively. A decrease in fertilization success was observed at concentrations as low as 0.5 cell mL-1 of Dinophysis spp. and 5 nM of PTX2, whereas no effect of OA could be observed. The effect on fertilization success was higher when both gamete types were concomitantly exposed compared to separate exposures, suggesting a synergistic effect. Our results also suggest that the effects could be due to cell-to-cell contact. These results highlight a potential effect of Dinophysis spp. and PTX2 on reproduction and recruitment of the Pacific oyster.