Influence of the semen quality of maintaining males of Atlantic salmon, Salmo salar, in sea water during the breeding season

International audience We have shown previously that the semen collected from male Atlantic salmon kept in sea water has often a low quality. To check for the hypothesis that this could be due to contamination by urine, we have compared the characteristics of semen collected by stripping, with those...

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Bibliographic Details
Main Authors: Maisse, Gérard, Billard, Roland, Cosson, J., Labbé, Catherine, Loir, Maurice, Le Gac, Florence, Fierville, F.
Other Authors: Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Institut National de la Recherche Agronomique (INRA), Station Marine, Université de Lille, Sciences et Technologies-PRES Université Lille Nord de France-Centre National de la Recherche Scientifique (CNRS), Muséum national d'Histoire naturelle (MNHN)
Format: Article in Journal/Newspaper
Language:French
Published: HAL CCSD 1998
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Online Access:https://hal.inrae.fr/hal-02698629
https://doi.org/10.1051/kmae:1998010
Description
Summary:International audience We have shown previously that the semen collected from male Atlantic salmon kept in sea water has often a low quality. To check for the hypothesis that this could be due to contamination by urine, we have compared the characteristics of semen collected by stripping, with those of semen collected directly in the deferent ducts, from males maintained in sea water or transferred to freshwater one month before. While the semen taken in the ducts of both batches of males (freshwater: ED;sea water : EM) has similar spermatocrit, motility, ATP content and fertility, these parameters were significantly lower for semen obtained by stripping from males in sea water. The analysis of the data suggests that some of these sperms were contaminated by urine and the hypothesis is proposed that the high magnesium content of urine could have activated the gametes, inducing a low ATP content and a low fertility. In addition, the fertility of frozen sperm from the ducts was significantly lower for the males in sea water as compared to the males in freshwater. The molar ratio cholesterol/phospholipids in the membranes of gametes did not differ for the two batches of semen. Lors d'une étude antérieure, nous avons montré que le maintien en mer des Saumons atlantiques mâles affecte la qualité du sperme. Pour tester l'hypothèse de l'influence néfaste d'une contamination par l'urine, nous avons comparé la qualité du sperme collecté par la technique classique (pression des flancs ou "stripping") à celle du sperme prélevé directement dans les canaux déférents, ceci chez des géniteurs, les uns gardés en mer et les autres transférés en eau douce un mois plus tôt. Alors que les spermes prélevés dans les canaux déférents des deux lots de mâles (eau douce : ED eau de mer : EM) ont un spermatocrite, une motilité, un contenu en ATP et une fécondance (en frais) peu différents, ces quatre paramètres sont significativement plus faibles pour les spermes obtenus par "stripping" des mâles en mer. L'analyse des données permet ...