New efficient recombinant expression system to engineer Candida antarctica lipase B
International audience Here, we report the use of Yarrowia lipolytica as a versatile expression host for developing protein engineering approaches to modify the properties of Candida antarctica lipase B. A reliable screening protocol was defined and validated using a saturation mutagenesis library,...
Published in: | Applied and Environmental Microbiology |
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Main Authors: | , , , , , , |
Other Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2010
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Subjects: | |
Online Access: | https://hal.inrae.fr/hal-02662242 https://doi.org/10.1128/AEM.03057-09 |
Summary: | International audience Here, we report the use of Yarrowia lipolytica as a versatile expression host for developing protein engineering approaches to modify the properties of Candida antarctica lipase B. A reliable screening protocol was defined and validated using a saturation mutagenesis library, yielding mutants displaying higher catalytic efficiencies than the wild-type enzyme. |
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