Efficiency of fluorescent multiplex polymerase chain reactions (PCRs) for rapid genotyping of harbour porpoises (Phocoena phocoena) with 11 microsatellite loci
International audience We developed two multiplex sets (PPH1 and PPH2) to amplify 11 polymorphic microsatellite loci previously used in harbour porpoise (Phocoena phocoena) studies with only four polymerase chain reactions (PCRs). PPH1 allows for the amplification of six loci at once, and PPH2 requi...
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Online Access: | https://hal.inrae.fr/hal-02657915 https://doi.org/10.1578/AM.32.3.2006.301 |
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ftccsdartic:oai:HAL:hal-02657915v1 2023-05-15T16:33:18+02:00 Efficiency of fluorescent multiplex polymerase chain reactions (PCRs) for rapid genotyping of harbour porpoises (Phocoena phocoena) with 11 microsatellite loci Fontaine, Michaël Galan, Maxime Bouquegneau, Jean Marie Michaux, Johan Centre de Biologie pour la Gestion des Populations (UMR CBGP) Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD France-Sud )-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) Laboratoire d'Océanologie (MARE Center) Université de Liège 2006 https://hal.inrae.fr/hal-02657915 https://doi.org/10.1578/AM.32.3.2006.301 en eng HAL CCSD European Association for Aquatic Mammals info:eu-repo/semantics/altIdentifier/doi/10.1578/AM.32.3.2006.301 hal-02657915 https://hal.inrae.fr/hal-02657915 doi:10.1578/AM.32.3.2006.301 PRODINRA: 17310 ISSN: 0167-5427 Aquatic Mammals https://hal.inrae.fr/hal-02657915 Aquatic Mammals, European Association for Aquatic Mammals, 2006, 32 (3), pp.301-304. ⟨10.1578/AM.32.3.2006.301⟩ PHOCOENA PHOCOENA HARBOUR PORPOISE CETACEAN MULTIPLEX-PCR MICROSATELLITE POPULATION GENETICS GENETIQUE DES POPULATIONS [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2006 ftccsdartic https://doi.org/10.1578/AM.32.3.2006.301 2021-10-16T23:58:37Z International audience We developed two multiplex sets (PPH1 and PPH2) to amplify 11 polymorphic microsatellite loci previously used in harbour porpoise (Phocoena phocoena) studies with only four polymerase chain reactions (PCRs). PPH1 allows for the amplification of six loci at once, and PPH2 requires three PCR reactions to amplify five loci. These two multiplex sets were tested on 100 animals from the Belgian coast and the Black Sea. They provided a rapid and efficient genotyping procedure for large-scale population genetic studies. Article in Journal/Newspaper Harbour porpoise Phocoena phocoena Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Aquatic Mammals 32 3 301 304 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
PHOCOENA PHOCOENA HARBOUR PORPOISE CETACEAN MULTIPLEX-PCR MICROSATELLITE POPULATION GENETICS GENETIQUE DES POPULATIONS [SDV]Life Sciences [q-bio] |
spellingShingle |
PHOCOENA PHOCOENA HARBOUR PORPOISE CETACEAN MULTIPLEX-PCR MICROSATELLITE POPULATION GENETICS GENETIQUE DES POPULATIONS [SDV]Life Sciences [q-bio] Fontaine, Michaël Galan, Maxime Bouquegneau, Jean Marie Michaux, Johan Efficiency of fluorescent multiplex polymerase chain reactions (PCRs) for rapid genotyping of harbour porpoises (Phocoena phocoena) with 11 microsatellite loci |
topic_facet |
PHOCOENA PHOCOENA HARBOUR PORPOISE CETACEAN MULTIPLEX-PCR MICROSATELLITE POPULATION GENETICS GENETIQUE DES POPULATIONS [SDV]Life Sciences [q-bio] |
description |
International audience We developed two multiplex sets (PPH1 and PPH2) to amplify 11 polymorphic microsatellite loci previously used in harbour porpoise (Phocoena phocoena) studies with only four polymerase chain reactions (PCRs). PPH1 allows for the amplification of six loci at once, and PPH2 requires three PCR reactions to amplify five loci. These two multiplex sets were tested on 100 animals from the Belgian coast and the Black Sea. They provided a rapid and efficient genotyping procedure for large-scale population genetic studies. |
author2 |
Centre de Biologie pour la Gestion des Populations (UMR CBGP) Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD France-Sud )-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) Laboratoire d'Océanologie (MARE Center) Université de Liège |
format |
Article in Journal/Newspaper |
author |
Fontaine, Michaël Galan, Maxime Bouquegneau, Jean Marie Michaux, Johan |
author_facet |
Fontaine, Michaël Galan, Maxime Bouquegneau, Jean Marie Michaux, Johan |
author_sort |
Fontaine, Michaël |
title |
Efficiency of fluorescent multiplex polymerase chain reactions (PCRs) for rapid genotyping of harbour porpoises (Phocoena phocoena) with 11 microsatellite loci |
title_short |
Efficiency of fluorescent multiplex polymerase chain reactions (PCRs) for rapid genotyping of harbour porpoises (Phocoena phocoena) with 11 microsatellite loci |
title_full |
Efficiency of fluorescent multiplex polymerase chain reactions (PCRs) for rapid genotyping of harbour porpoises (Phocoena phocoena) with 11 microsatellite loci |
title_fullStr |
Efficiency of fluorescent multiplex polymerase chain reactions (PCRs) for rapid genotyping of harbour porpoises (Phocoena phocoena) with 11 microsatellite loci |
title_full_unstemmed |
Efficiency of fluorescent multiplex polymerase chain reactions (PCRs) for rapid genotyping of harbour porpoises (Phocoena phocoena) with 11 microsatellite loci |
title_sort |
efficiency of fluorescent multiplex polymerase chain reactions (pcrs) for rapid genotyping of harbour porpoises (phocoena phocoena) with 11 microsatellite loci |
publisher |
HAL CCSD |
publishDate |
2006 |
url |
https://hal.inrae.fr/hal-02657915 https://doi.org/10.1578/AM.32.3.2006.301 |
genre |
Harbour porpoise Phocoena phocoena |
genre_facet |
Harbour porpoise Phocoena phocoena |
op_source |
ISSN: 0167-5427 Aquatic Mammals https://hal.inrae.fr/hal-02657915 Aquatic Mammals, European Association for Aquatic Mammals, 2006, 32 (3), pp.301-304. ⟨10.1578/AM.32.3.2006.301⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1578/AM.32.3.2006.301 hal-02657915 https://hal.inrae.fr/hal-02657915 doi:10.1578/AM.32.3.2006.301 PRODINRA: 17310 |
op_doi |
https://doi.org/10.1578/AM.32.3.2006.301 |
container_title |
Aquatic Mammals |
container_volume |
32 |
container_issue |
3 |
container_start_page |
301 |
op_container_end_page |
304 |
_version_ |
1766022999922704384 |