Efficiency of fluorescent multiplex polymerase chain reactions (PCRs) for rapid genotyping of harbour porpoises (Phocoena phocoena) with 11 microsatellite loci

International audience We developed two multiplex sets (PPH1 and PPH2) to amplify 11 polymorphic microsatellite loci previously used in harbour porpoise (Phocoena phocoena) studies with only four polymerase chain reactions (PCRs). PPH1 allows for the amplification of six loci at once, and PPH2 requi...

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Bibliographic Details
Published in:Aquatic Mammals
Main Authors: Fontaine, Michaël, Galan, Maxime, Bouquegneau, Jean Marie, Michaux, Johan
Other Authors: Centre de Biologie pour la Gestion des Populations (UMR CBGP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD France-Sud )-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Laboratoire d'Océanologie (MARE Center), Université de Liège
Format: Article in Journal/Newspaper
Language:English
Published: HAL CCSD 2006
Subjects:
PHOCOENA PHOCOENA
HARBOUR PORPOISE
CETACEAN
MULTIPLEX-PCR
MICROSATELLITE
POPULATION GENETICS
GENETIQUE DES POPULATIONS
[SDV]Life Sciences [q-bio]
Harbour porpoise
Phocoena phocoena
Online Access:https://hal.inrae.fr/hal-02657915
https://doi.org/10.1578/AM.32.3.2006.301
Description
Summary:International audience We developed two multiplex sets (PPH1 and PPH2) to amplify 11 polymorphic microsatellite loci previously used in harbour porpoise (Phocoena phocoena) studies with only four polymerase chain reactions (PCRs). PPH1 allows for the amplification of six loci at once, and PPH2 requires three PCR reactions to amplify five loci. These two multiplex sets were tested on 100 animals from the Belgian coast and the Black Sea. They provided a rapid and efficient genotyping procedure for large-scale population genetic studies.

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