A serological survey of anthrax in domestic dogs in Zimbabwe: a potential tool for anthrax surveillance

• Published in: Epidemiology and Infection
• Main Authors: Mukarati, N.L., Ndumnego, O., van Heerden, H., Ndhlovu, D. N., Matope, G., Caron, Alexandre, De Garine-Wichatitsky, M., Pfukenyi, D. M.
• Other Authors: University of Zimbabwe (UZ), University of Pretoria South Africa, Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE), Institut National de la Recherche Agronomique (INRA)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Faculdade de Veterinária, Universidade Federal do Ceará = Federal University of Ceará (UFC), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Kasetsart University (KU)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2018
• Subjects: Anthrax; domestic dogs; wildlife/livestock interface; Zimbabwe; seroprevalence; [SDV]Life Sciences [q-bio]; Canis lupus
• Online Access: https://hal.inrae.fr/hal-02622855; https://doi.org/10.1017/S0950268818001577

International audience Anthrax is an important disease caused by the bacterium Bacillus anthracis affecting both domestic and wild animals at the wildlife/livestock interface, defined here as a physical space in which wild and domestic species overlap in range and potentially interact. In endemic regions, sporadic anthrax outbreaks occur, causing significant deaths of both wildlife and livestock and sporadically, humans. However, it may also occur as isolated outbreaks with a few animals affected. Such isolated anthrax outbreaks maybe missed. High seroprevalence among carnivores suggests either regular non-fatal exposure to the pathogen circulating in a given environment, or contact with missed cases through consumption of anthrax carcases. To investigate the relevance of this potential indicator, a cross-sectional study was conducted to determine anthrax seroprevalence in domestic dogs (Canis lupus familiaris) from selected interface and non-interface areas of Zimbabwe with known history of anthrax outbreaks. Based on past anthrax outbreaks in the respective areas, the sites were further classified as high or low risk areas for anthrax outbreaks. Sera were collected from domestic dogs (n = 186) and tested for antibodies against B. anthracis protective antigens (PA) using an ELISA test. The overall seroprevalence was 51.6% (96/186; 95% CI 44.2-59.0). Sites from the non-interface areas recorded a significantly (P < 0.001) higher (72.1%) anthrax seroprevalence compared with those from the wildlife -livestock interface (41.5%). The results demonstrated a strong association (chi(2) = 14.3; OR = 3.2, 1.6 < OR < 6.2, P < 0.001) between anthrax seropositivity and interface type. Low-risk sites (42.5%) had a significantly (P = 0.044) lower seroprevalence compared with high-risk sites (58.5%) but still demonstrated high seroprevalence for areas where anthrax was last reported more than 20 years back. Dogs from Tsholotsho South were more than 90-times (OR = 96.5, 13.5 < OR < 690.8) more likely to be ...