Modeling of autophagy-related gene expression dynamics during long term fasting in European eel (Anguilla anguilla)

• Published in: Scientific Reports
• Main Authors: Bolliet, Valérie, Labonne, Jacques, Olazcuaga, Laure, Panserat, Stéphane, Seiliez, Iban
• Other Authors: Ecologie Comportementale et Biologie des Populations de Poissons (ECOBIOP), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Nutrition, Métabolisme, Aquaculture (NuMéA), This study was supported by the University of Pau et Pays de l’Adour (BQR MIRA “Etude de la mise en place des mécanismes de protéolyse chez la civelle d’anguille en période de jeûne. Relation avec leur comportement de migration estuarienne”
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2017
• Subjects: autophagy; eel; anguille; autophagie; jeune alimentaire; expression des gènes; [SDV.BA]Life Sciences [q-bio]/Animal biology; [SDE.BE]Environmental Sciences/Biodiversity and Ecology; Anguilla anguilla; European eel
• Online Access: https://hal.archives-ouvertes.fr/hal-01695895; https://hal.archives-ouvertes.fr/hal-01695895/document; https://hal.archives-ouvertes.fr/hal-01695895/file/2017_Bolliet_ScientificReports.pdf; https://doi.org/10.1038/s41598-017-18164-6

International audience Autophagy is an evolutionary conserved cellular self-degradation process considered as a major energy mobilizing system in eukaryotes. It has long been considered as a post-translationally regulated event, and the importance of transcriptional regulation of autophagy-related genes (atg) for somatic maintenance and homeostasis during long period of stress emerged only recently. In this regard, large changes in atg transcription have been documented in several species under diverse types of prolonged catabolic situations. However, the available data primarily concern atg mRNA levels at specific times and fail to capture the dynamic relationship between transcript production over time and integrated phenotypes. Here, we present the development of a statistical model describing the dynamics of expression of several atg and lysosomal genes in European glass eel (Anguilla anguilla) during long-term fasting at two temperatures (9 °C and 12 °C) and make use of this model to infer the effect of transcripts dynamics on an integrated phenotype – here weight loss. Our analysis shows long-term non-random fluctuating atg expression dynamics and reveals for the first time a significant contribution of atg transcripts production over time to weight loss. The proposed approach thus offers a new perspective on the long-term transcriptional control of autophagy and its physiological role.