Semi-pilot scale-up of a continuous packed-bed bioreactor system developed for the lipase-catalyzed production of pseudo-ceramides

• Published in: OCL
• Main Authors: Le Joubioux, Florian, Bridiau, Nicolas, Graber, Marianne, Maugard, Thierry
• Other Authors: LIttoral ENvironnement et Sociétés - UMRi 7266 (LIENSs), Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Génie Protéique et Cellulaire (LGPC), Université de La Rochelle (ULR)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2017
• Subjects: [SDV.BIO]Life Sciences [q-bio]/Biotechnology; Antarc*; Antarctica
• Online Access: https://hal.archives-ouvertes.fr/hal-01630116; https://hal.archives-ouvertes.fr/hal-01630116/document; https://hal.archives-ouvertes.fr/hal-01630116/file/OCL%20final.pdf; https://doi.org/10.1051/ocl/2017019

International audience Ceramides are sphingolipid compounds that are very attractive as active components in both the pharmaceutical and the cosmetic industries. In this study, the synthesis of 1-O,3-N-diacyl 3-amino-1,2-propanediol-type pseudo-ceramides was developed at the semi-pilot scale, starting from a two-step continuous enzymatic process with immobilized Candida antarctica lipase B (Novozym ® 435) in a packed-bed bioreactor, previously optimized at the laboratory scale. This process involved the selective N-acylation of 3-amino-1,2-propanediol (step 1), followed by the selective O-acylation of the N-acyl 3-amino-1,2-propanediol synthesized in the first step, with various fatty acids as acyl donors, to produce N,O-diacyl 3-amino-1,2-propanediol-type pseudo-ceramides (step 2). Under partially optimized operating conditions, high synthesis yields and production rates were obtained, within the ranges 76–92% and 3.7–4.6 g h À1 (step 1), or 23–36% and 1–1.4 g h À1 (step 2), respectively, depending on the fatty acids used as acyl donors. The overall synthesis yields varied from 20 to 33%: the best yield was obtained using palmitic acid and lauric acid as first and second acyl donors, respectively. Together with the high production rates also obtained with these acyl donors, this confirms that this two-step process has great potential for the production of differently functionalized 1-O,3-N-diacyl 3-amino-1,2-propanediol-type pseudo-ceramides on an industrial scale. Keywords: pseudo-ceramide / continuous bioprocess / packed-bed bioreactor / lipase / semi-pilot scale Passage à l'échelle semi-pilote d'un bioréacteur continu à lit fixe, développé pour la production de pseudo-céramides catalysée par une lipase. Les céramides sont des sphingolipides, composés actifs d'intérêt majeur pour les industries pharmaceutique et cosmétique. Lors de la présente étude, la synthèse de pseudo-céramides de type 1-O,3-N-diacyl 3-amino-1,2-propanediol a été développée à l'échelle semi-pilote, à partir d'un procédé enzymatique ...