Shell extracts of the edible mussel and oyster induce an enhancement of the catabolic pathway of human skin fibroblasts, in vitro

15 pages International audience Mollusc shells are composed of more than 95% calcium carbonate and less than 5% organic matrix consisting mostly of proteins, glycoproteins and polysaccharides. In this study, we investigated the effects of matrix macromolecular components extracted from the shells of...

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Published in:Cytotechnology
Main Authors: Latire, Thomas, Legendre, Florence, Bouyoucef, Mouloud, Marin, Frédéric, Carreiras, Franck, Rigot-Jolivet, Muriel, Lebel, Jean-Marc, Galéra, Philippe, Serpentini, Antoine
Other Authors: Université de Caen Normandie (UNICAEN), Normandie Université (NU), Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Microenvironnement cellulaire et pathologie (MILPAT), Normandie Université (NU)-Normandie Université (NU), Biogéosciences UMR 6282 Dijon (BGS), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Equipe de recherche sur les relations matrice extracellulaire-cellules (ERRMECe), Fédération INSTITUT DES MATÉRIAUX DE CERGY-PONTOISE (I-MAT), Université de Cergy Pontoise (UCP), Université Paris-Seine-Université Paris-Seine-Université de Cergy Pontoise (UCP), Université Paris-Seine-Université Paris-Seine, Department of Plastic and Reconstructive Surgery, St-Martin Clinic, Work financially supported by the ‘‘Fonds Unique Interministériel’’ (FUI, French ministry of Economy and Industry, SEMINEROIL program) 09 2 90 6042 , and by a PhD fellowship from the FUI and the Conseil Régional de Basse-Normandie 917RB103 .
Format: Article in Journal/Newspaper
Language:English
Published: HAL CCSD 2017
Subjects:
Shell matrix
Mollusc
Fibroblast
Extracellular matrix
Biological activity
[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Pacific
Crassostrea gigas
Pacific oyster
Online Access:https://hal.archives-ouvertes.fr/hal-01588926
https://doi.org/10.1007/s10616-017-0096-1
Description
Summary:15 pages International audience Mollusc shells are composed of more than 95% calcium carbonate and less than 5% organic matrix consisting mostly of proteins, glycoproteins and polysaccharides. In this study, we investigated the effects of matrix macromolecular components extracted from the shells of two edible molluscs of economic interest, i.e., the blue mussel Mytilus edulis and the Pacific oyster Crassostrea gigas. The potential biological activities of these organic molecules were analysed on human dermal fibroblasts in primary culture. Our results demonstrate that shell extracts of the two studied molluscs modulate the metabolic activities of the cells. In addition, the extracts caused a decrease of type I collagen and a concomitant increase of active MMP-1, both at the mRNA and the protein levels. Therefore, our results suggest that shell extracts from M. edulis and C. gigas contain molecules that promote the catabolic pathway of human dermal fibroblasts. This work emphasises the potential use of these shell matrices in the context of anti-fibrotic strategies, particularly against scleroderma. More generally, it stresses the usefulness to valorise bivalve shells that are coproducts of shellfish farming activity.

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