The paralytic shellfish toxin, saxitoxin, enters the cytoplasm and induces apoptosis of oyster immune cells through a caspase-dependent pathway

Article accepté Exposure of the toxin-producing dinoflagellate Alexandrium catenella (A. catenella) was previously demonstrated to cause apoptosis of hemocytes in the oyster species Crassostrea gigas. In this work, a coumarin-labeled saxitoxin appeared to spread throughout the cytoplasm of the hemoc...

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Bibliographic Details
Published in:Aquatic Toxicology
Main Authors: Abi-Khalil, Celina, Finkelstein, Darren S., Conejero, Geneviève, Du Bois, Justin, Destoumieux-Garzon, Delphine, Rolland, Jean Luc
Other Authors: Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Department of Chemistry, Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)
Format: Article in Journal/Newspaper
Language:English
Published: HAL CCSD 2017
Subjects:
Toxin
Harmful algae
Phytoplankton
Shellfish
crassostrea gigas
hémocyte
réaction hémocytaire
[SDV]Life Sciences [q-bio]
Crassostrea gigas
Online Access:https://hal.archives-ouvertes.fr/hal-01561503
https://hal.archives-ouvertes.fr/hal-01561503/document
https://hal.archives-ouvertes.fr/hal-01561503/file/Abi-KhalilC.-et%20al-PrePrint-AquaticToxico.-2017_%7B5391CD15-5587-4D24-A8CB-EEE879DFA9DB%7D.pdf
https://doi.org/10.1016/j.aquatox.2017.07.001
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Summary:Article accepté Exposure of the toxin-producing dinoflagellate Alexandrium catenella (A. catenella) was previously demonstrated to cause apoptosis of hemocytes in the oyster species Crassostrea gigas. In this work, a coumarin-labeled saxitoxin appeared to spread throughout the cytoplasm of the hemocytes. PSTs, including saxitoxin, were also shown to be directly responsible for inducing apoptosis in hemocytes, a process dependent on caspase activation and independent of reactive oxygen species (ROS) production. A series of in vitro labeling and microscopy experiments revealed that STX and analogs there of induced nuclear condensation, phosphatidylserine exposure, membrane permeability, and DNA fragmentation of hemocytes. Unlike in vertebrates, gonyautoxin-5 (GTX5), which ispresent in high concentrations in A. catenella, was found to be more toxic than saxitoxin (STX) to oyster immune cells. Altogether, results show that PSTs produced by toxic dinoflagellates enter the cytoplasm and induce apoptosis of oyster immune cells through a caspase-dependent pathway. Because of the central role of hemocytes in mollusc immune defense, PST-induced death of hemocytes could negatively affect resistance of bivalvemolluscs to microbial infection.

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