An improved PCR-based method for faster sex determination in brown trout [i](Salmo trutta)[/i] and Atlantic salmon [i](Salmo salar)[/i]

• Published in: Conservation Genetics Resources
• Main Authors: Quéméré, Erwan, Perrier, Charles, Besnard, Anne-Laure, Evanno, Guillaume, Baglinière, Jean-Luc, Guiguen, Yann, Launey, Sophie
• Other Authors: Unité de recherche Comportement et Ecologie de la Faune Sauvage (CEFS), Institut National de la Recherche Agronomique (INRA), Université Laval Québec (ULaval), Département de Biologie, Institut de Biologie Intégrative et des Systèmes Québec (IBIS), Écologie et santé des écosystèmes (ESE), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), ONEMA and the European Regional Development Fund (Transnational program Interreg IV, Project AARC—Atlantic Aquatic Resource Conservation)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2014
• Subjects: sdY; molecular sexing; archived tissues; salmo; brown trout; atlantic salmon; [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology; Atlantic salmon; Salmo salar
• Online Access: https://hal.archives-ouvertes.fr/hal-01205112; https://doi.org/10.1007/s12686-014-0259-8

The online version of this article (doi:10.1007/s12686-014-0259-8) contains supplementary material, which is available to authorized users Rapid and accurate sex-identification of salmonids has remained an important challenge for aquaculture and wild population study and management. A molecular method looking for the presence of the male-specific sdY gene has recently been proposed. However, the size of the amplified fragment (similar to 500pb) and the DNA revealing technique (agarose-gel electrophoresis) preclude its use on partially degraded DNA and in high-throughput population studies running smaller fragments on automated DNA analyzers. Here, we propose an improved molecular method based on the amplification of a shorter section of the sdY gene for sex-determination in Atlantic salmon and brown trout. Designed primers were multiplexed with several microsatellites and provided reliable sexing for 97 % of the individuals tested. We successfully applied this method to both fresh and archived tissues from 16 individuals (9 populations) of brown trout and 19 individuals (14 populations) of Atlantic salmon. This molecular marker also includes important length polymorphism providing useful Y-linked phylogeographic information.