RAD-seq mapping of spontaneous masculinization in XX doubled haploid rainbow trout lines

Sex in rainbow trout is primarily determined by a genetic male heterogametic system encoded by a master sex-determining gene sdY. However, the occurrence of spontaneous phenotypic males in all-female doubled haploid (DH) gynogenetic populations suggested that multiple sex-influencing loci could exis...

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Bibliographic Details
Main Authors: Guyomard, René, Guiguen, Yann, Bernard, Maria, Charlet, Aurélie, Dechamp, Nicolas, Hervet, Caroline, Chantry-Darmon, Céline, Krieg, Francine, Quillet, Edwige
Other Authors: Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Laboratoire d'Analyse Génétique pour les Espèces Animales (LABOGENA), Institut National de la Recherche Agronomique (INRA), ANR
Format: Conference Object
Language:English
Published: HAL CCSD 2014
Subjects:
salmonids
linkage map
sex determinism
[SDV]Life Sciences [q-bio]
Canada
Atlantic salmon
Online Access:https://hal.archives-ouvertes.fr/hal-01194148
https://hal.archives-ouvertes.fr/hal-01194148/document
https://hal.archives-ouvertes.fr/hal-01194148/file/2014_Guyomard_WCGALP_1.pdf
Description
Summary:Sex in rainbow trout is primarily determined by a genetic male heterogametic system encoded by a master sex-determining gene sdY. However, the occurrence of spontaneous phenotypic males in all-female doubled haploid (DH) gynogenetic populations suggested that multiple sex-influencing loci could exist in trout as found in other species (tilapia, common carp, Atlantic salmon, zebrafish). In order to get further insight in the genetic determinism of this un-expected masculinization, we crossed a DH XX spontaneously sex-reversed male with a normal female. Two F1 females were then reproduced by mitogynogenesis and their DH progeny (n=168) were genotyped using RAD sequencing. A genome scan of 9500 markers was obtained resulting in a linkage map of 2860 cM. Several putative QTL regions have been identified suggesting that more than one mutation could be involved in the observed deviation from the strict XX/XY sex determinism system.

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