New genetic markers to identify European resistant abalone to vibriosis revealed by high-resolution melting analysis, a sensitive and fast approach

• Published in: Marine Biology
• Main Authors: Meistertzheim, Anne-Leila, Calves, Isabelle, Roussel, Véronique, Van Wormhoudt, Alain, Laroche, Jean, Huchette, Sylvain, Paillard, Christine
• Other Authors: Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Centre de Formation et de Recherche sur les Environnements Méditérranéens (CEFREM), Université de Perpignan Via Domitia (UPVD)-Centre National de la Recherche Scientifique (CNRS), Station de Biologie Marine de Concarneau, Direction générale déléguée à la Recherche, à l’Expertise, à la Valorisation et à l’Enseignement-Formation (DGD.REVE), Muséum national d'Histoire naturelle (MNHN)-Muséum national d'Histoire naturelle (MNHN), Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Scea France Haliotis, ANR-08-VULN-0004,EVOLFISH,Effects of global warming and contamination on the evolutionnary response of two coastal fishes(2008), European Project: 222156,EC:FP7:SME,FP7-SME-2007-1,SUDEVAB(2008)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2014
• Subjects: OYSTER CRASSOSTREA-GIGAS; HALIOTIS-TUBERCULATA; SUMMER MORTALITY; IMMUNE-RESPONSE; LINKAGE MAP; SUSCEPTIBILITY; HARVEYI; IDENTIFICATION; INFECTION; SURVIVAL; ACL; [SDV]Life Sciences [q-bio]; [SDE.BE]Environmental Sciences/Biodiversity and Ecology; Crassostrea gigas
• Online Access: https://hal.univ-brest.fr/hal-01020946; https://doi.org/10.1007/s00227-014-2470-2

International audience Increasing temperature of seawater is often associated with increased exposure incidence of disease in field and in aquaculture populations. Numerous episodic mass mortalities of the abalone Haliotis tuberculata have been observed along the northern Brittany coast of France caused by a complex interaction between the host, pathogen and environmental factors. Here, we evaluated the potential of high-resolution melting (HRM) analysis for mutation genotyping and development of genetic markers for resistance to vibriosis in the gastropod species H. tuberculata. Small amplicon assays were developed and revealed genetic polymorphism between surviving and susceptible abalone obtained after two successive infections of aquaculture families in controlled conditions. Together with specific COI haplotypes, we identified particular genotypes in nascent polypeptide-associated complex subunit alpha and ferritin genes linked to the susceptibility or resistance of abalone to vibriosis. Selection of genitors based on these genes may increase the proportion in offspring of resistant individuals of more than 76 %. Finally, HRM assays constitute a very efficient genotyping tool to validate the genetic markers on a representative number of individuals of wild populations and thus identify future resistant genitors for aquaculture or conservation purposes