ESIEX-Electrical Swing Ion Exchange a Process Coupling Ion Exchange, Carbonic Acid Elution, and Electrochemical Regeneration

International audience A process which can be used for purification of a peptide is presented. This process is called ESIEX: Electrical Swing Ion Exchange. The principle of this process is based on a three steps cyclic mode, which avoids using pH buffers or generating wastes. The experimental set-up...

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Bibliographic Details
Published in:Separation Science and Technology
Main Authors: Lu, Wei, Grévillot, Georges, Muhr, Laurence
Other Authors: Laboratoire Réactions et Génie des Procédés (LRGP), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)
Format: Article in Journal/Newspaper
Language:English
Published: HAL CCSD 2011
Subjects:
carbonic acid
electrochemical regeneration
green eluent
peptide
AMINO-ACIDS
CHROMATOGRAPHY
DIOXIDE
[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering
Carbonic acid
Online Access:https://hal.archives-ouvertes.fr/hal-00776878
https://doi.org/10.1080/01496395.2011.585627
Description
Summary:International audience A process which can be used for purification of a peptide is presented. This process is called ESIEX: Electrical Swing Ion Exchange. The principle of this process is based on a three steps cyclic mode, which avoids using pH buffers or generating wastes. The experimental set-up consists of an electrodialysis apparatus in which the cells are filled with anion exchange resins. Carbon dioxide dissolved in water is used as "green eluent.'' Peptide is obtained pure. Assuming that equilibria in solution play a major role on the global separation factor compared to affinities for the support, peptides which can be fixed in a step but also eluted in another step should satisfy with a criterion: pK(a1) < pK(A-/A) < pK(a2). The peptide dissociation constant pK(A-/A) must be between the two dissociation constants of carbonic acid, that is, 6.35 and 10.35 (at 298 K).

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