High matrix interference affecting detection of PAH metabolites in bile of Atlantic hagfish () used for biomonitoring of deep-water oil production

International audience The characteristic biology and wide distribution of hagfish species makes them relevant for use in pollution biomonitoring at great water depths, particularly in regions where deep-water oil exploration may take place. The exposure of fish to petrogenic contaminants can normal...

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Bibliographic Details
Published in:Marine Environmental Research
Main Authors: Sundt, Rolf C., Beyer, Jonny, Vingen, Sjur, Sydnes, Magne O.
Other Authors: IRIS - International Research Institute of Stavanger, University of Stavanger
Format: Article in Journal/Newspaper
Language:English
Published: HAL CCSD 2011
Subjects:
Atlantic hagfish
bile fluorescence
PAH
biomonitoring
Barents Sea
[SDV]Life Sciences [q-bio]
Norway
Online Access:https://hal.archives-ouvertes.fr/hal-00703484
https://hal.archives-ouvertes.fr/hal-00703484/document
https://hal.archives-ouvertes.fr/hal-00703484/file/PEER_stage2_10.1016%252Fj.marenvres.2011.04.006.pdf
https://doi.org/10.1016/j.marenvres.2011.04.006
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Summary:International audience The characteristic biology and wide distribution of hagfish species makes them relevant for use in pollution biomonitoring at great water depths, particularly in regions where deep-water oil exploration may take place. The exposure of fish to petrogenic contaminants can normally be detected from the level of polycyclic aromatic hydrocarbon (PAH) metabolites in bile fluid. Some of these metabolites are strong fluorophores, allowing analytical detection by means of simple fluorometric techniques such as fixed wavelength fluorescence (FF) and synchronous fluorescence scanning (SFS). In the present study bile from Atlantic hagfish () collected in pristine areas (Barents Sea and southwestern Norway) displayed strong bile fluorescence levels, suggesting the presence of PAH contaminants. However, gas-chromatography-mass spectrometry (GC-MS) analyses ruled out PAHs as the origin for this fluorescence signal. Rather, the bile of contains components resulting in unusually strong background fluorescence interfering at the wavelength pairs used for detection of PAH metabolites. Possible background for the observed matrix interference and implications for detection of PAH metabolites in hagfish is discussed.

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