Non-negative Matrix Factorization under sparsity constraints to unmix in vivo spectrally resolved acquisitions

• Main Authors: Montcuquet, Anne-Sophie, Herve, Lionel, Navarro, Fabrice, Dinten, Jean-Marc, Mars, Jerome
• Other Authors: GIPSA - Signal Images Physique (GIPSA-SIGMAPHY), Département Images et Signal (GIPSA-DIS), Grenoble Images Parole Signal Automatique (GIPSA-lab), Université Pierre Mendès France - Grenoble 2 (UPMF)-Université Stendhal - Grenoble 3-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Pierre Mendès France - Grenoble 2 (UPMF)-Université Stendhal - Grenoble 3-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Grenoble Images Parole Signal Automatique (GIPSA-lab), Université Pierre Mendès France - Grenoble 2 (UPMF)-Université Stendhal - Grenoble 3-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Pierre Mendès France - Grenoble 2 (UPMF)-Université Stendhal - Grenoble 3-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS), Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)
• Format: Conference Object
• Language: English
• Published: HAL CCSD 2010
• Subjects: [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing; [INFO.INFO-TS]Computer Science [cs]/Signal and Image Processing; Iceland
• Online Access: https://hal.archives-ouvertes.fr/hal-00625417; https://hal.archives-ouvertes.fr/hal-00625417/document; https://hal.archives-ouvertes.fr/hal-00625417/file/whispers2010_submission_55.pdf

International audience Fluorescence imaging in diffusive media is an emerging imaging modality for medical applications which uses injected fluorescent markers (several ones may be simultaneously injected) that bind to specific targets, as tumors. The region of interest is illuminated with near infrared light and the emitted back fluorescence is analyzed to localize the fluorescence sources. To investigate thick medium, as the fluorescence signal decreases with the light travel distance, any disturbing signal, such as biological tissues intrinsic fluorescence - called autofluorescence -, is a limiting factor. To remove autofluorescence and isolate each specific fluorescent signal from the others, a spectroscopic approach, based on Non-negative Matrix Factorization, is explored. We ran an NMF algorithm with sparsity constraints on experimental data, and successfully obtained separated in vivo fluorescence spectra.