Comparative analysis of Vibrio splendidus-related strains isolated during Crassostrea gigas mortality events

• Main Authors: Le Roux, Frédérique, Gay, Mélanie, Lambert, Christophe, Waechter, Magali, Poubalanne, Saravanne, Chollet, Bruno, Nicolas, Jean-Louis, Berthe, Franck
• Other Authors: Laboratoire de Génétique et Pathologie (LGP), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Université européenne de Bretagne - European University of Brittany (UEB), Écloserie Grainocéan, Physiologie et Ecophysiologie des Mollusques Marins (PE2M)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2002
• Subjects: Oyster mortality; PCR-RFLP; Epidemiology; Virulence; Vibrio splendidus; [SDV.BA]Life Sciences [q-bio]/Animal biology; Pacific; Crassostrea gigas
• Online Access: https://hal.archives-ouvertes.fr/hal-00616793; https://hal.archives-ouvertes.fr/hal-00616793/document; https://hal.archives-ouvertes.fr/hal-00616793/file/Le_Roux_et_al_2002_Aquat_Living_Resour_15_251-258_-auteurs.pdf

International audience French mollusc production is based mainly on the Pacific cupped oyster, Crassostrea gigas. Since 1991, annual mass mortality of juveniles has been reported during summer months. These recurring episodes concern professionals who fear that like Portugese oyster, C. angulata, C. gigas could in turn disappear following one of these epizooties. Previously, bacteriological analysis of moribund oyster juveniles yielded an isolate of a Vibrio splendidus biovar II strain, named TNEMF6. This isolate was demonstrated to be pathogenic to Crassostrea gigas spat by experimental challenge. To study the association between summer oyster mortality and presence of TNEMF6 cluster strains, Vibrionaceae fauna were isolated from infected spat along the French Atlantic coast between 1997-1998. Strains related to V. splendidus biovar II were selected. Comparison with TNEMF6 was performed by classical biochemical tests and polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) of SSU rDNA, rpoD, and gyrB genes. Genomic similarities were confirmed by DNA/DNA hybridization. Only one strain out of 14, TNNIII7, was found to be closely related to the pathogenic bacteria. Neither the phenotypic nor the genotypic markers used in this study were able to distinguish pathogenic from non-pathogenic strains of the widespread V. splendidus. However, future genetic comparisons of TNEMF6 and TNNIII7 is likely to reveal genes involved in pathogenicity.