Cytochrome P450 1A1 expression in cetacean skin biopsies from the Indian Ocean

International audience The study describes cytochrome P-450 1A1 (CYPA1) expression in the skin of different cetacean species (Megaptera novaeangliae, n= 15; Stenella attenuata, n=7 and Stenella longirostris, n= 24) from the Mozambique Channel island of Mayotte. Immunohistochemical examination was pe...

Full description

Bibliographic Details
Published in:Marine Pollution Bulletin
Main Authors: Jauniaux, Thierry, Farnir, Frédéric, Fontaine, Michaël, Kiszka, Jeremy, Sarlet, Michael, Coignoul, Freddy
Other Authors: Faculté de Médecine Vétérinaire Liège, Département de Biostatistiques, Université de Liège, Ecologie Systématique et Evolution (ESE), Université Paris-Sud - Paris 11 (UP11)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS), LIttoral ENvironnement et Sociétés - UMRi 7266 (LIENSs), Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS), Département de Pathologie Générale, Faculté de médecine vétérinaire, Département de Pathotologie Générale
Format: Article in Journal/Newspaper
Language:English
Published: HAL CCSD 2011
Subjects:
Cytochrome P450
Biopsy
Skin
Immunohistochemistry
Mayotte
PORPOISES PHOCOENA-PHOCOENA
BOTTLE-NOSED-DOLPHIN
TURSIOPS-TRUNCATUS
INTEGUMENT
MARINE MAMMALS
CONTAMINANTS
POLLUTANTS
INDUCTION
EXPOSURE
[SDV.EE.ECO]Life Sciences [q-bio]/Ecology
environment/Ecosystems
Indian
Megaptera novaeangliae
Phocoena phocoena
Online Access:https://hal.archives-ouvertes.fr/hal-00608678
https://hal.archives-ouvertes.fr/hal-00608678/document
https://hal.archives-ouvertes.fr/hal-00608678/file/CytochromeLast.pdf
https://doi.org/10.1016/j.marpolbul.2011.04.005
Description
Summary:International audience The study describes cytochrome P-450 1A1 (CYPA1) expression in the skin of different cetacean species (Megaptera novaeangliae, n= 15; Stenella attenuata, n=7 and Stenella longirostris, n= 24) from the Mozambique Channel island of Mayotte. Immunohistochemical examination was performed with a monoclonal antibody against scup cytochrome CYPA1. The sex was determined using a molecular approach consisting in the genotyping sex-specific genes. CYPA1 was detected at the junction between epidermis and blubber on dolphins only, mostly in the endothelial cells. Similar observation was obtained in the dermis of one M. novaeangliae. Immunohistochemical slides were scored to evaluate the expression of the CYPA1 and a higher expression was observed in S. longirostris, suggesting a higher exposure to pollutants for this species. The difference of expression between sexes was not significant.

Similar Items