Structural organization and sequence analysis of the globin locus in Atlantic salmon.

• Main Authors: McMorrow, T., Wagner, A., Deryckere, F., Gannon, F.
• Other Authors: Department of Microbiology (NATIONAL DIAGNOSTICS CENTRE), University College Galway
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 1996
• Subjects: MESH: Animals; MESH: Base Sequence; MESH: Multigene Family; MESH: Promoter Regions (Genetics); MESH: Restriction Mapping; MESH: Salmon; MESH: Sequence Analysis; DNA; MESH: Sequence Homology; Amino Acid; MESH: Binding Sites; MESH: Cloning; Molecular; MESH: DNA Footprinting; MESH: Deoxyribonuclease I; MESH: Genes; MESH: Globins; MESH: Hydrogen-Ion Concentration; MESH: Molecular Sequence Data; [SDV.BC]Life Sciences [q-bio]/Cellular Biology; Atlantic salmon
• Online Access: https://hal.archives-ouvertes.fr/hal-00151177

Preliminary analysis of Atlantic salmon alpha- and beta-globin genes indicated that these genes are linked in a 3' to 3' orientation, with the RNA-coding sequences located on opposite strands. In this report, we show that two different alpha-globin genes have the same orientation and are encoded on the same strand whereas two different beta-globin genes are encoded on the opposite strand and also have the same orientation. This cluster of globin genes is divided into two subclusters: one for the Bohr globin genes and one for the non-Bohr globin genes. This is the first evidence for this type of arrangement found for globin genes. DNase I footprint analysis of two of the globin promoters show erythroid-specific transcription factor binding sites that have also been found in human and other mammalian globin genes.