| Summary: | Study and use of metal-containing biological reagents often involves the need to manipulate the redox level. We report herein the synthesis of a heterogeneous catalyst system that allows the use of H₂ as a reductant for the one-electron reduction of horseheart ferricytochrome c (cyt cₒₓ), sperm whale myoglobin, and stellacyanin from the lacquer of Rhus vernicifera. Application of the principles illustrated by our catalyst in other systems is possible inasmuch as the reducing power of H₂ is sufficiently great that many biological systems are thermodynamically reducible with H₂. An advantage in using H₂ as a source of reducing power is that the oxidation product is H⺠which is acceptable since most biological systems are studied in buffered media. A heterogeneous catalyst is desirable to facilitate the separation of the catalyst from the product. © 1981 American Chemical Society. N.S.L. acknowledges support as a John and Fannie Hertz Fellow, 1977-1981. D.C.B. acknowledges partial support as an M.I.T. NPW Fellow, 1981. Partial support from the United States Department of Energy, Basic Energy Sciences, Division of Chemical Sciences, is acknowledged. We appreciate the generous assistance of Professor Edward I. Solomon and his research group and the use of their purified stellacyanin. Helpful discussions with Professors William H. Orme-Johnson, Alexander Klibanov, and George M. Whitesides are gratefully acknowledged.
|
|---|