Microbial Survival Strategies In Ancient Permafrost: Insights From Metagenomics
In permafrost (perennially frozen ground) microbes survive oligotrophic conditions, sub-zero temperatures, low water availability and high salinity over millennia. Viable life exists in permafrost tens of thousands of years old but we know little about the metabolic and physiological adaptations to...
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ftcalifstateuniv:oai:dspace.calstate.edu:10211.3/204654 2023-05-15T17:55:52+02:00 Microbial Survival Strategies In Ancient Permafrost: Insights From Metagenomics Mackelprang, Rachel Burkert, Alexander Haw, Monica Mahendrarajah, Tara Conaway, Christopher H Douglas, Thomas A Waldrop, Mark P 2017 http://hdl.handle.net/10211.3/204654 en eng Natural Publishing Group International Society for Microbial Ecology doi.org/10.1038/ismej.2017.93 The ISME Journal 11, 2305-2318. (2017) 1751-7362 http://hdl.handle.net/10211.3/204654 Bacteria Freezing Metagenomics Microbial Viability Permafrost Phylogeny Salinity Temperature Article 2017 ftcalifstateuniv https://doi.org/10.1038/ismej.2017.93 2022-04-13T11:41:11Z In permafrost (perennially frozen ground) microbes survive oligotrophic conditions, sub-zero temperatures, low water availability and high salinity over millennia. Viable life exists in permafrost tens of thousands of years old but we know little about the metabolic and physiological adaptations to the challenges presented by life in frozen ground over geologic time. In this study we asked whether increasing age and the associated stressors drive adaptive changes in community composition and function. We conducted deep metagenomic and 16 S rRNA gene sequencing across a Pleistocene permafrost chronosequence from 19 to 33 years before present (kyr). We found that age markedly affected community composition and reduced diversity. Reconstruction of paleovegetation from metagenomic sequence suggests vegetation differences in the paleo record are not responsible for shifts in community composition and function. Rather, we observed shifts consistent with long-term survival strategies in extreme cryogenic environments. These include increased reliance on scavenging detrital biomass, horizontal gene transfer, chemotaxis, dormancy, environmental sensing and stress response. Our results identify traits that may enable survival in ancient cryoenvironments with no influx of energy or new materials. Article in Journal/Newspaper permafrost California State University (CSU): DSpace The ISME Journal 11 10 2305 2318 |
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Open Polar |
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California State University (CSU): DSpace |
op_collection_id |
ftcalifstateuniv |
language |
English |
topic |
Bacteria Freezing Metagenomics Microbial Viability Permafrost Phylogeny Salinity Temperature |
spellingShingle |
Bacteria Freezing Metagenomics Microbial Viability Permafrost Phylogeny Salinity Temperature Mackelprang, Rachel Burkert, Alexander Haw, Monica Mahendrarajah, Tara Conaway, Christopher H Douglas, Thomas A Waldrop, Mark P Microbial Survival Strategies In Ancient Permafrost: Insights From Metagenomics |
topic_facet |
Bacteria Freezing Metagenomics Microbial Viability Permafrost Phylogeny Salinity Temperature |
description |
In permafrost (perennially frozen ground) microbes survive oligotrophic conditions, sub-zero temperatures, low water availability and high salinity over millennia. Viable life exists in permafrost tens of thousands of years old but we know little about the metabolic and physiological adaptations to the challenges presented by life in frozen ground over geologic time. In this study we asked whether increasing age and the associated stressors drive adaptive changes in community composition and function. We conducted deep metagenomic and 16 S rRNA gene sequencing across a Pleistocene permafrost chronosequence from 19 to 33 years before present (kyr). We found that age markedly affected community composition and reduced diversity. Reconstruction of paleovegetation from metagenomic sequence suggests vegetation differences in the paleo record are not responsible for shifts in community composition and function. Rather, we observed shifts consistent with long-term survival strategies in extreme cryogenic environments. These include increased reliance on scavenging detrital biomass, horizontal gene transfer, chemotaxis, dormancy, environmental sensing and stress response. Our results identify traits that may enable survival in ancient cryoenvironments with no influx of energy or new materials. |
format |
Article in Journal/Newspaper |
author |
Mackelprang, Rachel Burkert, Alexander Haw, Monica Mahendrarajah, Tara Conaway, Christopher H Douglas, Thomas A Waldrop, Mark P |
author_facet |
Mackelprang, Rachel Burkert, Alexander Haw, Monica Mahendrarajah, Tara Conaway, Christopher H Douglas, Thomas A Waldrop, Mark P |
author_sort |
Mackelprang, Rachel |
title |
Microbial Survival Strategies In Ancient Permafrost: Insights From Metagenomics |
title_short |
Microbial Survival Strategies In Ancient Permafrost: Insights From Metagenomics |
title_full |
Microbial Survival Strategies In Ancient Permafrost: Insights From Metagenomics |
title_fullStr |
Microbial Survival Strategies In Ancient Permafrost: Insights From Metagenomics |
title_full_unstemmed |
Microbial Survival Strategies In Ancient Permafrost: Insights From Metagenomics |
title_sort |
microbial survival strategies in ancient permafrost: insights from metagenomics |
publisher |
Natural Publishing Group |
publishDate |
2017 |
url |
http://hdl.handle.net/10211.3/204654 |
genre |
permafrost |
genre_facet |
permafrost |
op_relation |
doi.org/10.1038/ismej.2017.93 The ISME Journal 11, 2305-2318. (2017) 1751-7362 http://hdl.handle.net/10211.3/204654 |
op_doi |
https://doi.org/10.1038/ismej.2017.93 |
container_title |
The ISME Journal |
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11 |
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10 |
container_start_page |
2305 |
op_container_end_page |
2318 |
_version_ |
1766163900701605888 |