Detection of Avian Pneumovirus in Wild Canada Geese (Branta canadensis) and Blue-Winged Teal (Anas discors)

Choanal cleft swab samples from 770 wild Canada geese (Branta canadensis) and 358 blue-winged teal (Anas discors), captured for relocation or banding, were examined for the presence of avian pneumovirus (APV) RNA by reverse transcription (RT)–polymerase chain reaction (PCR) and for virus isolation....

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Bibliographic Details
Main Authors: R. S. Bennett, B. McComb, H-J. Shin, M. K. Njenga, K. V. Nagaraja, D. A. Halvorson
Format: Text
Language:English
Published: American Association of Avian Pathologists 2002
Subjects:
Canada
Branta canadensis
Canada Goose
Online Access:https://doi.org/10.1637/0005-2086(2002)046[1025:DOAPIW]2.0.CO;2
Description
Summary:Choanal cleft swab samples from 770 wild Canada geese (Branta canadensis) and 358 blue-winged teal (Anas discors), captured for relocation or banding, were examined for the presence of avian pneumovirus (APV) RNA by reverse transcription (RT)–polymerase chain reaction (PCR) and for virus isolation. The swab samples were pooled into groups of 5 or 10.Sixty eight of 102 (66.7%) pooled goose samples were RT-PCR positive for APV RNA. Thirteen of 52 (25.0%) pooled blue-winged teal samples were RT-PCR positive for APV RNA. APV RNA-positive samples were inoculated onto chick embryo fibroblasts (CEF) and QT-35 cells. Infectious APV was isolated from five Canada goose pooled samples in CEF and from one Canada goose pool in QT-35 cells but not from blue-winged teal.

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