Effectiveness of glucose–methanol extender forcryopreservation of Huso huso spermatozoa

• Published in: Animal Reproduction Science
• Main Authors: آراملی, محمد صادق, گلشاهی, کریم, نظری, رجب محمد, آراملی, سلیم, بنان, اشکان
• Format: Text
• Language: English
• Published: Elsevier 1394
• Subjects: QT فیزیولوژی; WP بیماریهای زنان; Beluga; Beluga*
• Online Access: https://eprints.arums.ac.ir/6978/; https://eprints.arums.ac.ir/6978/1/1-s2.0-S0378432015300166-main2.pdf; http://www.sciencedirect.com/science/article/pii/S0378432015300166; https://doi.org/10.1016/j.anireprosci.2015.09.005

The present approach was designed to evaluate the methanol–glucose extender effects onsperm cryopreservation in beluga sturgeon, Huso huso. Sperm quality was examined bymeasuring post-thaw sperm motility and fertilizing rate at hatching stage. We first testedthe effect of glucose concentration (0, 0.10, 0.15, 0.20 and 0.30 M) in a methanol extender onpost-thaw sperm motility. The optimal cryopreservation conditions were found to be 0.2 Mglucose in the extender. Then, motility and fertilization rates of sperm cryopreserved with0.2 M glucose and 10% methanol (GM) were compared to Tris–sucrose–KCl in 10% methanolextender (TSKM). Additionally, sperm motility and fertilizing ability in relation to 15 and30 min equilibration in GM extender before and after cryopreservation were measured.Higher post-thaw sperm motility duration and percentage as well as fertilization rate wereobtained with the GM extender when compared to TSKM extender. Equilibration of sperm inextender did not affect the motility quality of either fresh-diluted or frozen/thawed sperm,while fertilization rate showed a significant decline alone after 30 min of post-thaw storage.Our results indicated that the use of a simple extender consisting of 0.2 M glucose in 10%methanol can be an alternative cryopreservation method to those previously described forsturgeons.