hTERT CIRCULATING DNA AS AN INDICATOR FOR GASTRIC AND ESOPHAGEAL CANCERS IN ARDABIL, IRAN
Background: In Iran, Gastric Cancer (GC) is the most common cancer in the men and has been detected as the third among woman. Ardabil province, in northwestern Iran, has the highest rate of GC in this country. Esophageal Cancer (EC) is a relatively rare form of cancer, but some world areas have a ma...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Text |
| Language: | Persian |
| Published: |
1390
|
| Subjects: | |
| Online Access: | https://eprints.arums.ac.ir/3469/ https://eprints.arums.ac.ir/3469/1/cancer.pdf http://worldgicancer.com/wcgi/wgic2011/index.html |
| Summary: | Background: In Iran, Gastric Cancer (GC) is the most common cancer in the men and has been detected as the third among woman. Ardabil province, in northwestern Iran, has the highest rate of GC in this country. Esophageal Cancer (EC) is a relatively rare form of cancer, but some world areas have a markedly higher incidence than others: Belgium, China, Iran, Iceland, India, Japan, the United Kingdom, appear to have a higher incidence, as well as the region around the Caspian Sea. The American Cancer Society estimates that during 2007, approximately 15,560 new esophageal cancer cases will be diagnosed in the United States. Esophageal cancer is the 6th most common cause of cancer death in the world. Among last years, circulating nucleic acids on serum and plasma have been detected as an indicator in some cancers. In the present investigation, detecting the human TElomerase Reverse Transcriptase (hTERT) DNA as catalytic subunit of telomerase on serum of cancer affected patients compared to control group has been studied. Methods: DNA extraction was carried out on serum samples obtained from 100 primary GC affected patients, 100 primary EC affected patients and 100 participants with any detected cancer affecting persons in their 1st to 3rd degree of relatives as control group for each type of cancer. Quantitation detecting the hTERT DNA was performed by the TaqMan assay on Real time PCR system by using appropriate serial dilutions of genomic DNA to create standard curve. Results: Qualitative findings showed hTERT DNA presentation in 45.4%, 68.5%, and 23.9% of GC group, EC group, and controls, respectively. Those tend to significant associations between hTERT DNA in the serum and GC and EC in our province. Quantitative amounts of detected hTERT DNA were associated with gastric and esophageal carcinomas and the tumors pathologic indicators as well. Conclusion: Finding prognostic factors by non-invasive assays could help us to manage the therapeutic and diagnostic procedures, especially for cancers with low survival such ... |
|---|