Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the Pacific oyster Crassostrea gigas
We report the development of 18 new polymorphic microsatellite DNA markers derived from Crassostrea gigas expressed sequences tags. Genotyping of 48 wild adult oysters sampled from Marennes-OlEron bay (France) revealed 12 to 48 alleles per locus. Observed and expected heterozygosity levels ranged fr...
Published in: | Molecular Ecology Resources |
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Language: | English |
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Wiley / Blackwell
2009
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Online Access: | https://archimer.ifremer.fr/doc/2009/publication-6419.pdf https://doi.org/10.1111/j.1755-0998.2009.02525.x https://archimer.ifremer.fr/doc/00000/6419/ |
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ftarchimer:oai:archimer.ifremer.fr:6419 2023-05-15T15:57:04+02:00 Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the Pacific oyster Crassostrea gigas Sauvage, Christopher Boudry, Pierre Lapegue, Sylvie 2009-05 application/pdf https://archimer.ifremer.fr/doc/2009/publication-6419.pdf https://doi.org/10.1111/j.1755-0998.2009.02525.x https://archimer.ifremer.fr/doc/00000/6419/ eng eng Wiley / Blackwell https://archimer.ifremer.fr/doc/2009/publication-6419.pdf doi:10.1111/j.1755-0998.2009.02525.x https://archimer.ifremer.fr/doc/00000/6419/ 2009 Wiley Blackwell Publishing, Inc. info:eu-repo/semantics/openAccess restricted use Molecular Ecology Resources (1755-098X) (Wiley / Blackwell), 2009-05 , Vol. 9 , N. 3 , P. 853-855 Pacific oyster Null alleles EST SSR markers Crassostrea gigas text Publication info:eu-repo/semantics/article 2009 ftarchimer https://doi.org/10.1111/j.1755-0998.2009.02525.x 2021-09-23T20:17:15Z We report the development of 18 new polymorphic microsatellite DNA markers derived from Crassostrea gigas expressed sequences tags. Genotyping of 48 wild adult oysters sampled from Marennes-OlEron bay (France) revealed 12 to 48 alleles per locus. Observed and expected heterozygosity levels ranged from 0.64 to 1 and from 0.77 to 0.97, respectively. The development of these new markers creates a useful complementary tool for population genetics studies, parentage analysis and mapping in Pacific oyster, a species of major aquacultural and ecological importance. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Molecular Ecology Resources 9 3 853 855 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Pacific oyster Null alleles EST SSR markers Crassostrea gigas |
spellingShingle |
Pacific oyster Null alleles EST SSR markers Crassostrea gigas Sauvage, Christopher Boudry, Pierre Lapegue, Sylvie Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the Pacific oyster Crassostrea gigas |
topic_facet |
Pacific oyster Null alleles EST SSR markers Crassostrea gigas |
description |
We report the development of 18 new polymorphic microsatellite DNA markers derived from Crassostrea gigas expressed sequences tags. Genotyping of 48 wild adult oysters sampled from Marennes-OlEron bay (France) revealed 12 to 48 alleles per locus. Observed and expected heterozygosity levels ranged from 0.64 to 1 and from 0.77 to 0.97, respectively. The development of these new markers creates a useful complementary tool for population genetics studies, parentage analysis and mapping in Pacific oyster, a species of major aquacultural and ecological importance. |
format |
Article in Journal/Newspaper |
author |
Sauvage, Christopher Boudry, Pierre Lapegue, Sylvie |
author_facet |
Sauvage, Christopher Boudry, Pierre Lapegue, Sylvie |
author_sort |
Sauvage, Christopher |
title |
Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the Pacific oyster Crassostrea gigas |
title_short |
Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the Pacific oyster Crassostrea gigas |
title_full |
Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the Pacific oyster Crassostrea gigas |
title_fullStr |
Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the Pacific oyster Crassostrea gigas |
title_full_unstemmed |
Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the Pacific oyster Crassostrea gigas |
title_sort |
identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the pacific oyster crassostrea gigas |
publisher |
Wiley / Blackwell |
publishDate |
2009 |
url |
https://archimer.ifremer.fr/doc/2009/publication-6419.pdf https://doi.org/10.1111/j.1755-0998.2009.02525.x https://archimer.ifremer.fr/doc/00000/6419/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Molecular Ecology Resources (1755-098X) (Wiley / Blackwell), 2009-05 , Vol. 9 , N. 3 , P. 853-855 |
op_relation |
https://archimer.ifremer.fr/doc/2009/publication-6419.pdf doi:10.1111/j.1755-0998.2009.02525.x https://archimer.ifremer.fr/doc/00000/6419/ |
op_rights |
2009 Wiley Blackwell Publishing, Inc. info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1111/j.1755-0998.2009.02525.x |
container_title |
Molecular Ecology Resources |
container_volume |
9 |
container_issue |
3 |
container_start_page |
853 |
op_container_end_page |
855 |
_version_ |
1766392701280845824 |