Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the Pacific oyster Crassostrea gigas

• Published in: Molecular Ecology Resources
• Main Authors: Sauvage, Christopher, Boudry, Pierre, Lapegue, Sylvie
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley / Blackwell 2009
• Subjects: Pacific oyster; Null alleles; EST SSR markers; Crassostrea gigas; Pacific
• Online Access: https://archimer.ifremer.fr/doc/2009/publication-6419.pdf; https://doi.org/10.1111/j.1755-0998.2009.02525.x; https://archimer.ifremer.fr/doc/00000/6419/

We report the development of 18 new polymorphic microsatellite DNA markers derived from Crassostrea gigas expressed sequences tags. Genotyping of 48 wild adult oysters sampled from Marennes-OlEron bay (France) revealed 12 to 48 alleles per locus. Observed and expected heterozygosity levels ranged from 0.64 to 1 and from 0.77 to 0.97, respectively. The development of these new markers creates a useful complementary tool for population genetics studies, parentage analysis and mapping in Pacific oyster, a species of major aquacultural and ecological importance.