Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the Pacific oyster Crassostrea gigas

We report the development of 18 new polymorphic microsatellite DNA markers derived from Crassostrea gigas expressed sequences tags. Genotyping of 48 wild adult oysters sampled from Marennes-OlEron bay (France) revealed 12 to 48 alleles per locus. Observed and expected heterozygosity levels ranged fr...

Full description

Bibliographic Details
Published in:Molecular Ecology Resources
Main Authors: Sauvage, Christopher, Boudry, Pierre, Lapegue, Sylvie
Format: Article in Journal/Newspaper
Language:English
Published: Wiley / Blackwell 2009
Subjects:
Pacific oyster
Null alleles
EST SSR markers
Crassostrea gigas
Pacific
Online Access:https://archimer.ifremer.fr/doc/2009/publication-6419.pdf
https://doi.org/10.1111/j.1755-0998.2009.02525.x
https://archimer.ifremer.fr/doc/00000/6419/
Description
Summary:We report the development of 18 new polymorphic microsatellite DNA markers derived from Crassostrea gigas expressed sequences tags. Genotyping of 48 wild adult oysters sampled from Marennes-OlEron bay (France) revealed 12 to 48 alleles per locus. Observed and expected heterozygosity levels ranged from 0.64 to 1 and from 0.77 to 0.97, respectively. The development of these new markers creates a useful complementary tool for population genetics studies, parentage analysis and mapping in Pacific oyster, a species of major aquacultural and ecological importance.

Similar Items