Oyster hemocytes express a proline-rich peptide displaying synergistic antimicrobial activity with a defensin

A cDNA sequence that encodes a 61-amino acid polypeptide precursor with homologies to proline-rich antimicrobial peptides (AMPs) was identified in the oyster Crassostrea gigas. After release of a hydrophobic signal peptide. the resulting 37-amino acid peptide, Cg-Prp, is composed of an acidic region...

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Bibliographic Details
Published in:Molecular Immunology
Main Authors: Gueguen, Yannick, Romestand, Bernard, Fievet, Julie, Schmitt, Paulina, Destoumieux Garzon, Delphine, Franck, Vandenbulcke, Philippe, Bulet, Bachere, Evelyne
Format: Article in Journal/Newspaper
Language:English
Published: Elsevier 2009
Subjects:
Antimicrobial peptide
Mollusk
Synergy
Invertebrate
Innate immunity
Pacific oyster
Pacific
Crassostrea gigas
Online Access:https://archimer.ifremer.fr/doc/2009/publication-6232.pdf
https://doi.org/10.1016/j.molimm.2008.07.021
https://archimer.ifremer.fr/doc/00000/6232/
Description
Summary:A cDNA sequence that encodes a 61-amino acid polypeptide precursor with homologies to proline-rich antimicrobial peptides (AMPs) was identified in the oyster Crassostrea gigas. After release of a hydrophobic signal peptide. the resulting 37-amino acid peptide, Cg-Prp, is composed of an acidic region and a cationic proline-rich region. To evaluate the biological properties of Cg-Prp, multiple proline-rich peptides corresponding to putative processing of the full-length Cg-Prp were synthesized. A limited antimicrobial activity was observed for two of them, which also showed strong synergistic antimicrobial activity with Cg-Def, a defensin from C gigas. To our knowledge, this is the first evidence of synergy between a defensin and another AMP in an invertebrate. By in situ hybridization, the expression of Cg-prp was found to be restricted to hemocytes and induced following bacterial challenge. Cg-prp transcripts were also detected in hemocytes infiltrating mantle, where Cg-Def is expressed. Additionally, by immunocytochemistry, we showed that Cg-Prp or one of its variants is present in some hemocytes together with defensins. In conclusion, we described here the first proline-rich AMP from mollusk. From our study, it is likely to provide a first line of defense against bacterial invasion by acting through synergy with defensins. (C) 2008 Elsevier Ltd. All rights reserved.

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