Vibrio crassostreae sp nov., isolated from the haemolymph of oysters (Crassostrea gigas)

Polyphasic analysis of five new Vibrio isolates originating from the haernolymph of diseased cultured oysters is described. The new isolates were closely related to Vibrio splendidus, having 98 % 16S rRNA gene sequence similarity. gyrB phylogenetic analysis, fluorescent amplified-fragment length pol...

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Bibliographic Details
Published in:International Journal of Systematic and Evolutionary Microbiology
Main Authors: Faury, Nicole, Saulnier, Denis, Thompson, F, Gay, Melanie, Swings, J, Leroux, Frederique
Format: Article in Journal/Newspaper
Language:English
Published: Society for General Microbiology 2004
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Online Access:https://archimer.ifremer.fr/doc/2004/publication-591.pdf
https://doi.org/10.1099/ijs.0.63232-0
https://archimer.ifremer.fr/doc/00000/591/
Description
Summary:Polyphasic analysis of five new Vibrio isolates originating from the haernolymph of diseased cultured oysters is described. The new isolates were closely related to Vibrio splendidus, having 98 % 16S rRNA gene sequence similarity. gyrB phylogenetic analysis, fluorescent amplified-fragment length polymorphism (FAFLP) fingerprinting and DNA-DNA hybridization experiments clearly showed that the new isolates form a tight genomic group that is different from the currently known Vibrio species. It is proposed to accommodate these isolates in a novel species, Vibrio crassostreae sp. nov. (type strain LGP 7(T) =LMG 22240(T)=Clp 108327(T)). Phenotypic and chemotaxonomic features that differentiate V. crassostreae from other known Vibrio species include arginine dihydrolase, utilization and fermentation of various carbon sources, beta-galactosidase activity, NO2 production and the presence of the fatty acids 14: 0 iso and 16: 0 iso.