Development of a duplex Taqman real-time PCR assay for rapid identification of Vibrio splendidus -related and V. aestuarianus strains from bacterial cultures

To enable the rapid and accurate identification of Vibrio splendidus-related and V. aestuarianus strains associated with Pacific cupped oyster Crassostrea gigas mortality, we developed a duplex Taqman real-time PCR assay and evaluated its efficacy. This technique proved to be rapid, sensitive, and s...

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Bibliographic Details
Published in:Journal of Microbiological Methods
Main Authors: Saulnier, Denis, De Decker, Sophie, Tourbiez, Delphine, Travers, Marie-agnes
Format: Article in Journal/Newspaper
Language:English
Published: Elsevier Science Bv 2017
Subjects:
Multiplex real-time PCR
Gene sequencing
Molluscs
Splendidus Glade
Vibrio aestuarianus
Pacific
Crassostrea gigas
Online Access:https://archimer.ifremer.fr/doc/00392/50308/51077.pdf
https://doi.org/10.1016/j.mimet.2017.07.002
https://archimer.ifremer.fr/doc/00392/50308/
Description
Summary:To enable the rapid and accurate identification of Vibrio splendidus-related and V. aestuarianus strains associated with Pacific cupped oyster Crassostrea gigas mortality, we developed a duplex Taqman real-time PCR assay and evaluated its efficacy. This technique proved to be rapid, sensitive, and specific and will be particularly valuable for epidemiologic studies.

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