Short-term storage and cryopreservation of turbot (Scophthalmus maximus) sperm

Short-term storage over several days as well as cryopreservation of turbot (Scophthalmus maximus) sperm were studied. Two extenders, Ringer 200 and artificial seminal liquid (ASL2), are suggested for semen collection in order to avoid the deleterious effect of urine contamination, and for the purpos...

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Bibliographic Details
Main Authors: Chereguini, Olvido, Cal, Rosa Maria, Dreanno, Catherine, Ogier De Baulny, Bénédicte, Suquet, Marc, Maisse, Gérard
Format: Article in Journal/Newspaper
Language:English
Published: Gauthier-villars 1997
Subjects:
turbot
sperm
storage
cryopreservation
Ringer
Scophthalmus maximus
Turbot
Online Access:https://archimer.ifremer.fr/doc/00176/28724/27173.pdf
https://doi.org/10.1051/alr:1997028
https://archimer.ifremer.fr/doc/00176/28724/
Description
Summary:Short-term storage over several days as well as cryopreservation of turbot (Scophthalmus maximus) sperm were studied. Two extenders, Ringer 200 and artificial seminal liquid (ASL2), are suggested for semen collection in order to avoid the deleterious effect of urine contamination, and for the purpose of short-term storage between 0 and 15 degrees C. Oxygen atmosphere is not suitable for turbot sperm storage. Turbot spermatozoa undergo cryopreservation with a high rate of success especially in a sucrose solution with 10% dimethyl sulfoxide (DMSO) and 10% egg yolk.

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