Long-term effects of the cryopreservation of turbot (Psetta maxima) spermatozoa

• Published in: Aquatic Living Resources
• Main Authors: Suquet, Marc, Dreanno, Catherine, Petton, Bruno, Normant, Yvon, Omnes, Marie-helene, Billard, Roland
• Format: Article in Journal/Newspaper
• Language: English
• Published: Gauthier-villars/editions Elsevier 1998
• Subjects: turbot; Psetta maxima; sperm; cryopreservation; hatching; Turbot
• Online Access: https://archimer.ifremer.fr/doc/00176/28721/27176.pdf; https://doi.org/10.1016/S0990-7440(99)80030-8; https://archimer.ifremer.fr/doc/00176/28721/

The survival of turbot eggs and the rearing capacities of larvae stemmed from artificial fertilization practices using frozen-thawed spermatozoa were evaluated. Furthermore, the viability of sperm samples stored during a 9 month period in liquid nitrogen was assessed. No significant difference in the fertilization rate, hatching rate, survival and wet weight of 10-day old larvae were observed using fresh or frozen-thawed spermatozoa. The motility recorded at 10 s and 60 s post-activation and the fertilization capacity of frozen-thawed spermatozoa were not significantly decreased during a 9 month storage period in liquid nitrogen. These results confirm the high quality of the turbot spermatozoa stemmed from the cryopreservation process, allowing their use for routine aquaculture practices.