Experimental ostreid herpesvirus 1 infection of the Pacific oyster Crassostrea gigas: Kinetics of virus DNA detection by q-PCR in seawater and in oyster samples

Herpes- and herpes-like viruses are known to infect a wide range of bivalve mollusc species throughout the world. Abnormal summer mortalities associated to the detection of ostreid herpesvirus 1 (OsHV-1) have been currently reported in France among larvae and spat of the Pacific cupped oyster Crasso...

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Bibliographic Details
Published in:Virus Research
Main Authors: Schikorski, David, Faury, Nicole, Pepin, Jean-francois, Saulnier, Denis, Tourbiez, Delphine, Renault, Tristan
Format: Article in Journal/Newspaper
Language:English
Published: Elsevier Science Bv 2011
Subjects:
Ostreid herpesvirus 1
Cupped Pacific oyster
Crassostrea gigas
Experimental infection
Kinetic viral DNA quantification
Pacific
Pacific oyster
Online Access:https://archimer.ifremer.fr/doc/00025/13607/10688.pdf
https://doi.org/10.1016/j.virusres.2010.07.031
https://archimer.ifremer.fr/doc/00025/13607/
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Summary:Herpes- and herpes-like viruses are known to infect a wide range of bivalve mollusc species throughout the world. Abnormal summer mortalities associated to the detection of ostreid herpesvirus 1 (OsHV-1) have been currently reported in France among larvae and spat of the Pacific cupped oyster Crassostrea gigas. In the present work, we have developed an experimental protocol of horizontal transmission based on the cohabitation between healthy and experimentally infected oysters. Through a cohabitation trial, the kinetics of OsHV-1 detection in different oyster organs and seawater samples were investigated and characterized for the first time using real time quantitative PCR.

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