New Structure of Aeromonas salmonicida O-Polysaccharide Isolated from Ill Farmed Fish

• Published in: Microorganisms
• Main Authors: Ucieklak, Karolina, Wojtys-Tekiel, Sylwia, Leroy, Garance, Le Devendec, Laëtitia, Baron, Sandrine, Kaszowska, Marta
• Other Authors: Polska Akademia Nauk = Polish Academy of Sciences = Académie polonaise des sciences (PAN), Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Ploufragan-Plouzané-Niort ANSES, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), This study was financed through the «ANTIBIOFISH» research project supported by both French ministries in charge of environment and agriculture. This project is part of the national effort to reduce antimicrobial resistance in veterinary medicine called “EcoAntibio2017”. This work was supported by the Polish National Agency for Academic Exchange (NAWA), mobility grant number PPN/BFR/2020/1/00031 and 46907VD-PHC Polonium 2021. This work was supported by the statutory funds of the Laboratory of Microbial Immunochemistry and Vaccines of the Hirszfeld Institute of Immunology & Experimental Therapy, Polish Academy of Sciences.
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2024
• Subjects: Aeromonas salmonicida; O-polysaccharide diversity; HR MAS NMR; lipopolysaccharide; [SDV]Life Sciences [q-bio]; Turbot
• Online Access: https://anses.hal.science/anses-04693995; https://doi.org/10.3390/microorganisms12081575

International audience The diversity of O-polysaccharides (O-antigens) among 28 Aeromonas salmonicida strains isolated from ill fish has been determined by using high-resolution magic angle spinning (HR MAS) NMR spectroscopy. The new O-polysaccharide has been identified in two isolates. This new structure was investigated by 1H and 13C NMR spectroscopy and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The following structure of the linear hexasaccharide repeating unit of A. salmonicida O-antigen has been established: →3)-α-L-Rhap-(1→3)-α-D-ManpNAc-(1→2)-β-D-Glcp-(1→3)-α-L-Rhap2OAc4OAc-(1→3)-β-D-ManpNAc-(1→3)-α-D-Glcp-(1→. This new A. salmonicida O-polysaccharide was detected among two isolates collected from trout and turbot fish in 2010 and 2011, respectively. Further investigations should be conducted to evaluate the distribution of this new O-polysaccharide among a larger collection of isolates, depending on their geographic origin, the species of fish, and the health status of the fish.