Aldehyde perception induces specific molecular responses in Laminaria digitata and affects algal consumption by a specialist grazer

• Published in: The Plant Journal
• Main Authors: Xing, Qikun, Cabioch, Léa, Desrut, Antoine, Le Corguillé, Gildas, Rousvoal, Sylvie, Dartevelle, Laurence, Rolland, Elodie, Guitton, Yann, Potin, Philippe, Markov, Gabriel, V, Faugeron, Sylvain, Leblanc, Catherine
• Other Authors: Laboratoire de Biologie Intégrative des Modèles Marins (LBI2M), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff = Roscoff Marine Station (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie Intégrative (LBI), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Fédération de recherche de Roscoff (FR2424), Station biologique de Roscoff = Roscoff Marine Station (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), ABiMS - Informatique et bioinformatique = Analysis and Bioinformatics for Marine Science (ABIMS), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff = Roscoff Marine Station (SBR), Laboratoire d'étude des Résidus et Contaminants dans les Aliments (LABERCA), École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Plateforme Metabolomics, Lipidomics and Steroidomics Analysis (MELISA), École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-MetaboHUB-Grand-Ouest, MetaboHUB-MetaboHUB, Centro de conservacion marina and CeBiB, Pontificia Universidad Católica de Chile (UC), Station biologique de Roscoff Roscoff (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Végétaux marins et biomolécules, Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-GOEMAR-Centre National de la Recherche Scientifique (CNRS), CNRS, Sorbonne Université, Région Bretagne, ANR-11-INBS-0010,METABOHUB,Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation(2011)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2023
• Subjects: Brown algae; biotic interactions; defense and signaling responses; transcriptomics; metabolomics; Brown algae biotic interactions defense and signaling responses transcriptomics metabolomics; [SDV.BV]Life Sciences [q-bio]/Vegetal Biology; [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry; Molecular Biology/Genomics [q-bio.GN]; North Atlantic
• Online Access: https://hal.sorbonne-universite.fr/hal-04197531; https://hal.sorbonne-universite.fr/hal-04197531/document; https://hal.sorbonne-universite.fr/hal-04197531/file/The%20Plant%20Journal%20-%202023%20-%20Xing.pdf; https://doi.org/10.1111/tpj.16450

International audience SUMMARY In the marine environment, distance signaling based on water‐borne cues occurs during interactions between macroalgae and herbivores. In the brown alga Laminaria digitata from North‐Atlantic Brittany, oligoalginates elicitation or grazing was shown to induce chemical and transcriptomic regulations, as well as emission of a wide range of volatile aldehydes, but their biological roles as potential defense or warning signals in response to herbivores remain unknown. In this context, bioassays using the limpet Patella pellucida and L. digitata were carried out for determining the effects of algal transient incubation with 4‐hydroxyhexenal (4‐HHE), 4‐hydroxynonenal (4‐HNE) and dodecadienal on algal consumption by grazers. Simultaneously, we have developed metabolomic and transcriptomic approaches to study algal molecular responses after treatments of L. digitata with these chemical compounds. The results indicated that, unlike the treatment of the plantlets with 4‐HNE or dodecadienal, treatment with 4‐HHE decreases algal consumption by herbivores at 100 ng.ml −1 . Moreover, we showed that algal metabolome was significantly modified according to the type of aldehydes, and more specifically the metabolite pathways linked to fatty acid degradation. RNAseq analysis further showed that 4‐HHE at 100 ng.ml −1 can activate the regulation of genes related to oxylipin signaling pathways and specific responses, compared to oligoalginates elicitation. As kelp beds constitute complex ecosystems consisting of habitat and food source for marine herbivores, the algal perception of specific aldehydes leading to targeted molecular regulations could have an important biological role on kelps/grazers interactions.