Environmental microplastics disrupt swimming activity in acute exposure in Danio rerio larvae and reduce growth and reproduction success in chronic exposure in D. rerio and Oryzias melastigma

• Published in: Environmental Pollution
• Main Authors: Cormier, Bettie, Cachot, Jérôme, Blanc, Mélanie, Cabar, Mathieu, Clérandeau, Christelle, Dubocq, Florian, Le Bihanic, Florane, Morin, Bénédicte, Zapata, Sarah, Bégout, Marie-Laure, Cousin, Xavier
• Other Authors: Environnements et Paléoenvironnements OCéaniques (EPOC), Observatoire aquitain des sciences de l'univers (OASU), Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École Pratique des Hautes Études (EPHE), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Örebro University, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), MARine Biodiversity Exploitation and Conservation - Station Ifremer Palavas (UMR MARBEC PALAVAS), MARine Biodiversity Exploitation and Conservation - MARBEC (UMR MARBEC ), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), This work was developed under the EPHEMARE project (Ecotoxicological effects of microplastics in marine ecosystems), supported by national funding agencies within the framework of JPI Oceans (FCT JPIOCEANS/0005/2015; FORMAS, 2015-01865; ANR-15-JOCE-0002-01). Bettie Cormier was directly supported by an IdEx grant from the University of Bordeaux., ANR-15-JOCE-0002,EPHEMARE,Ecotoxicological effects of microplastics in marine ecosystems(2015)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2022
• Subjects: Environmental microplastics; Fish; Reprotoxicity; Growth and behaviour alterations; Biomarkers; Adsorbed chemicals; [SDV]Life Sciences [q-bio]; [SDV.TOX]Life Sciences [q-bio]/Toxicology; North Atlantic
• Online Access: https://hal.inrae.fr/hal-03776197; https://hal.inrae.fr/hal-03776197/document; https://hal.inrae.fr/hal-03776197/file/cormier_2022_environ_pollut.pdf; https://doi.org/10.1016/j.envpol.2022.119721

International audience Microplastics (MPs), widely present in aquatic ecosystems, can be ingested by numerous organisms, but their toxicity remains poorly understood. Toxicity of environmental MPs from 2 beaches located on the Guadeloupe archipelago, Marie Galante (MG) and Petit-Bourg (PB) located near the North Atlantic gyre, was evaluated. A first experiment consisted in exposing early life stages of zebrafish (Danio rerio) to MPs at 1 or 10 mg/L. The exposure of early life stages to particles in water induced no toxic effects except a decrease in larval swimming activity for both MPs exposures (MG or PB). Then, a second experiment was performed as a chronic feeding exposure over 4 months, using a freshwater fish species, zebrafish, and a marine fish species, marine medaka (Oryzias melastigma). Fish were fed with food supplemented with environmentally relevant concentrations (1% wet weight of MPs in food) of environmental MPs from both sites. Chronic feeding exposure led to growth alterations in both species exposed to either MG or PB MPs but were more pronounced in marine medaka. Ethoxyresorufin-O-deethylase (EROD) and acetylcholinesterase (AChE) activities were only altered for marine medaka. Reproductive outputs were modified following PB exposure with a 70 and 42% decrease for zebrafish and marine medaka, respectively. Offspring of both species (F1 generation) were reared to evaluate toxicity following parental exposure on unexposed larvae. For zebrafish offspring, it revealed premature mortality after parental MG exposure and parental PB exposure produced behavioural disruptions with hyperactivity of F1 unexposed larvae. This was not observed in marine medaka offspring. This study highlights the ecotoxicological consequences of short and long-term exposures to environmental microplastics relevant to coastal marine areas, which represent essential habitats for a wide range of aquatic organisms.