| Summary: | RESULTS OBTAINED: Chile is heavily dependent on agriculture; therefore, it is important to improve agricultural management to ensure its sustainability. Improved agricultural practices can not only reduce the negative health and environmental impacts of agriculture, but also open access to new, more profitable markets that require high levels of food safety and pesticide traceability (Coria and Elgueta, 2022). Concerns about the quality and safety of products consumed in Chile have increased due to reports showing poor use of pesticides. Pesticide exposure affects the health not only of farm workers but also of people from the general population. Environmental exposure to organophosphate pesticides occurs, for example, through pesticide residues in fruits and vegetables, spraying near farmland, and pesticide residues in water and soil. Improvements in the regulation and control of pesticide use are clearly needed (Coria and Elgueta, 2022; Ciper, 2022). Results obtained: (Specific Objective 1) Quality assurance of method was carried out following the SANTE 12682/2019 guidelines. The guidelines establish that lettuce and chard corresponding to the same group of Leafy Vegetables. Standard ISO/IEC 17025:2017 was implemented. The accuracy was expressed as percentage of recovery, the precision as repeatability, the limit of detection (LOD), the limit of quantification (LOQ) and curves of calibration were used for the validation process (Table 1). The LOQ was be defined as the lowest concentration that could be quantified with an acceptable recovery (70-120%), started at 0.01 mg kg-1 and further increased depending on the pesticide. The relative standard deviation (RSD, %) was determined by analyzing replicate samples at three concentration levels (n = 5/level) and set at <20%). Quantification was performed using a mixture of known concentrations of the pesticides in a blank matrix and the area of the corresponding peak in the sample compared with the one of the known standards. The recoveries for all vegetables ...
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