Optimised scat collection protocols for dietary DNA metabarcoding in vertebrates

See the referenced paper for more information. These spreadsheets provide the sequences counts for the DNA groups found in the scats of Shy Albatross at Albatross Island, Tasmania, Australia. Samples were collected during three stages of the breeding season: Incubation (Late September 2014), Brood (...

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Bibliographic Details
Other Authors: AADC (originator), AU/AADC > Australian Antarctic Data Centre, Australia (resourceProvider)
Format: Dataset
Language:unknown
Published: Australian Ocean Data Network
Subjects:
biota
oceans
ALBATROSSES/PETRELS AND ALLIES
EARTH SCIENCE
BIOLOGICAL CLASSIFICATION
ANIMALS/VERTEBRATES
BIRDS
DNA METABARCODING
MISEQ GENOME SEQUENCING
SHY ALBATROSS
DIET
THALASSARCHE CAUTA
ALBATROSS ISLAND
AMD/AU
AMD
CEOS
GEOGRAPHIC REGION &gt
POLAR
OCEAN &gt
SOUTHERN OCEAN
CONTINENT &gt
AUSTRALIA/NEW ZEALAND &gt
AUSTRALIA &gt
Tasmania
Albatross Island
New Zealand
Southern Ocean
Online Access:https://researchdata.ands.org.au/optimised-scat-collection-metabarcoding-vertebrates/755481
https://data.aad.gov.au/metadata/records/AAS_4014_McInnes_et_al_2016
http://data.aad.gov.au/aadc/portal/download_file.cfm?file_id=4660
https://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4014_McInnes_et_al_2016
https://secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=AAS_4014
Description
Summary:See the referenced paper for more information. These spreadsheets provide the sequences counts for the DNA groups found in the scats of Shy Albatross at Albatross Island, Tasmania, Australia. Samples were collected during three stages of the breeding season: Incubation (Late September 2014), Brood (December 2014) and Chick-rearing (March 2014 and 2015). Scat samples were collected from breeding birds, chicks and non-breeders. 'Breeders' were identified as individuals incubating an egg or attending a chick, whereas 'non-breeders' were usually pairs that had reoccupied the colony and were building new practice nests with no chick present. Non-breeders in the colony include immature birds that have not yet bred and mature birds of breeding age that did not breed in a particular season (e.g. no partner or insufficient body condition) Details of the molecular methods and synthesis of this data can be found in: McInnes, J.C., Alderman, R., Deagle, B.E. Lea, M-A., Raymond, B. and Jarman, S.N. 2016. Optimised scat collection protocols for dietary DNA metabarcoding in vertebrates. Methods in Ecology and Evolution. Samples were collected to determine the affect that sample age, collection substrate, breeding stage and incubation duration had on the DNA amplification success and the proportion of food DNA. Using these data, optimised scat collection protocols were developed.

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