Real-time PCR measurements of alkB in SAB contaminated sediment (SRE4 experiment).

Real-time PCR assays were used to measure the potential of the microbial population to degrade alkanes - the principal component of Special Antarctic Blend diesel. This was done by using a real-time PCR assay to measure the levels of alkane mono-oxygenase genes (alkB). In this work, the assays were...

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Bibliographic Details
Other Authors: BOWMAN, JOHN (hasPrincipalInvestigator), POWELL, SHANE (processor), Australian Antarctic Data Centre (publisher)
Format: Dataset
Language:unknown
Published: Australian Antarctic Data Centre
Subjects:
environment
OIL SPILLS
EARTH SCIENCE
HUMAN DIMENSIONS
ENVIRONMENTAL IMPACTS
quantitative real-time PCR
alkane mono-oxygenase
special Antarctic blend diesel
ADS &gt
Automated DNA Sequencer
LABORATORY
CONTINENT &gt
ANTARCTICA
GEOGRAPHIC REGION &gt
POLAR
Antarctic
Windmill Islands
Casey Station
O'Brien Bay
Antarc*
Antarctica
Online Access:https://researchdata.ands.org.au/real-time-pcr-sre4-experiment/701810
https://doi.org/10.26179/5d3e7b2896119
https://data.aad.gov.au/metadata/records/SRE4_alkB_allyears
http://nla.gov.au/nla.party-617536
Description
Summary:Real-time PCR assays were used to measure the potential of the microbial population to degrade alkanes - the principal component of Special Antarctic Blend diesel. This was done by using a real-time PCR assay to measure the levels of alkane mono-oxygenase genes (alkB). In this work, the assays were used to determine the levels of these genes in sediment exposed to SAB and control sediments from the SRE4 in-situ biodegradation experiment. The samples used in this experiment were collected from O'Brien Bay, near Casey Station in the Windmill Islands. The data in this record is for all time points in the experiment. The download file contains a readme which provides further information about the dataset, as well as an excel and csv copy of the data. This work was completed as part of ASAC project 2672 (ASAC_2672).

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