terminal restriction fragment length polymorphism analysis of 16S rRNA and alkane-monooxygenase genes in Macquarie Island Soils

• Other Authors: POWELL, SHANE (hasPrincipalInvestigator), POWELL, SHANE (processor), BOWMAN, JOHN (hasPrincipalInvestigator), Australian Antarctic Data Centre (publisher)
• Format: Dataset
• Language: unknown
• Published: Australian Antarctic Data Centre
• Subjects: environment; TERRESTRIAL ECOSYSTEMS; EARTH SCIENCE; BIOSPHERE; OIL SPILLS; HUMAN DIMENSIONS; ENVIRONMENTAL IMPACTS; SOILS; LAND SURFACE; COMMUNITY STRUCTURE; ECOLOGICAL DYNAMICS; COMMUNITY DYNAMICS; microbial ecology; 16S rRNA; alkane mono-oxygenase; terminal Restriction Fragment Length Polymorphism; biodiversity; FIELD SURVEYS; LABORATORY; FIELD INVESTIGATION; OCEAN > SOUTHERN OCEAN > MACQUARIE ISLAND; Southern Ocean; Macquarie Island
• Online Access: https://researchdata.ands.org.au/terminal-restriction-fragment-island-soils/701402; https://doi.org/10.4225/15/59b1f93941f41; https://data.aad.gov.au/metadata/records/MI08_trflp; http://nla.gov.au/nla.party-617536

Samples were collected on Macquarie Island from three sites: the main powerhouse, the fuel farm and a reference site on the isthmus. The microbial communities in these samples were analysed by terminal-restriction fragment length polymorphism of both the 16S rRNA and alkane mono-oxygenase genes. The data consists of two excel files, one containing the 16S rRNA gene information and one containing the alkane mono-oxygenase results. The first sheet in each workbook contains experimental procedure details. The data itself consists of a matrix of terminal restriction fragment (tRF) size (in basepairs) by sample containing the percentage peak area of each tRF. This work was carried out as part of AAS project 2672.