terminal restriction fragment length polymorphism analysis of 16S rRNA and alkane-monooxygenase genes in Macquarie Island Soils

Samples were collected on Macquarie Island from three sites: the main powerhouse, the fuel farm and a reference site on the isthmus. The microbial communities in these samples were analysed by terminal-restriction fragment length polymorphism of both the 16S rRNA and alkane mono-oxygenase genes. The...

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Bibliographic Details
Other Authors: POWELL, SHANE (hasPrincipalInvestigator), POWELL, SHANE (processor), BOWMAN, JOHN (hasPrincipalInvestigator), Australian Antarctic Data Centre (publisher)
Format: Dataset
Language:unknown
Published: Australian Antarctic Data Centre
Subjects:
environment
TERRESTRIAL ECOSYSTEMS
EARTH SCIENCE
BIOSPHERE
OIL SPILLS
HUMAN DIMENSIONS
ENVIRONMENTAL IMPACTS
SOILS
LAND SURFACE
COMMUNITY STRUCTURE
ECOLOGICAL DYNAMICS
COMMUNITY DYNAMICS
microbial ecology
16S rRNA
alkane mono-oxygenase
terminal Restriction Fragment Length Polymorphism
biodiversity
FIELD SURVEYS
LABORATORY
FIELD INVESTIGATION
OCEAN &gt
SOUTHERN OCEAN &gt
MACQUARIE ISLAND
Southern Ocean
Macquarie Island
Online Access:https://researchdata.ands.org.au/terminal-restriction-fragment-island-soils/701402
https://doi.org/10.4225/15/59b1f93941f41
https://data.aad.gov.au/metadata/records/MI08_trflp
http://nla.gov.au/nla.party-617536
Description
Summary:Samples were collected on Macquarie Island from three sites: the main powerhouse, the fuel farm and a reference site on the isthmus. The microbial communities in these samples were analysed by terminal-restriction fragment length polymorphism of both the 16S rRNA and alkane mono-oxygenase genes. The data consists of two excel files, one containing the 16S rRNA gene information and one containing the alkane mono-oxygenase results. The first sheet in each workbook contains experimental procedure details. The data itself consists of a matrix of terminal restriction fragment (tRF) size (in basepairs) by sample containing the percentage peak area of each tRF. This work was carried out as part of AAS project 2672.

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