| Summary: | Maintenance and Update Frequency: notPlanned Credit Webster, Nicole S, Dr (Principal Investigator) The structure and composition of microbial communities inhabiting the soft coral Alcyonium antarcticum were investigated across 3 differentially contaminated sites within McMurdo Sound, Antarctica - McMurdo Station (MM), Scott Base (SB) and Cape Armitage (CA). \n \nSamples from all sites were subjected to culture-based analysis, denaturing gradient gel electrophoresis (DGGE), 16S rRNA gene clone-library analysis, and (FISH). \n \nPhylogenetic groups included Gamma-, Alpha- and Beta- proteobacteria; Bacteroidetes; Firmicutes; Actinomycetales; Planctomycetes; and Chlorobi and bacteria from the functional group of sulfate-reducing bacteria.\n To investigate the structure and composition of microbial communities inhabiting the soft coral Alcyonium antarcticum. \n \nTo examine any variation within and between Alcyonium antarcticum bacterial communities from 3 differentially contaminated sites within McMurdo Sound, Antarctica .\n This was the first investigation of microbial communities associated with Antarctic soft corals. \n \nAccession numbers assigned to sequencing products: Culture1-Culture10 (DG312224, DG312225, DG312226, DG312227, DG312228, DG312229, DG312230, DG312231, DG312232, DG312233); OTU1-OTU15 (DG312234, DG312235, DG312236, DG312237, DG312238, DG312239, DG312240, DG312241, DG312242, DG312243, DG312244, DG312245, DG312246, DG312247, DG312248); DGGE Band1-Band11, (DG312249, DG312250, DG312251, DG312252, DG312253, DG312254, DG312255, DG312256, DG312257, DG312258) and Band14-Band16 (DG312259, DG312260, DG312261).\n
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