Molecular Characterisation of Cold-Adapted Metallo-Oxotransferases from the Dimethylsulfoxide Reductase Family Expressed by Antarctic Bacteria
The latitudes and longitudes provided in spatial coverage are taken from the metadata records for ASAC projects 708 and 1012. Metadata record for data from ASAC Project 2547 See the link below for public details on this project. Pue (greater than 90% as determined by SDS-PAGE) samples of nitrate red...
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Australian Ocean Data Network
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Online Access: | https://researchdata.ands.org.au/molecular-characterisation-cold-antarctic-bacteria/686536 https://data.aad.gov.au/metadata/records/ASAC_2547 https://data.aad.gov.au/eds/2997/download http://www.ncbi.nlm.nih.gov/genbank/ https://secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=2547 http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=ASAC_2547 |
Summary: | The latitudes and longitudes provided in spatial coverage are taken from the metadata records for ASAC projects 708 and 1012. Metadata record for data from ASAC Project 2547 See the link below for public details on this project. Pue (greater than 90% as determined by SDS-PAGE) samples of nitrate reductase have been isolated from the Antarctic bacterium, Shewanella gelidimarina (ACAM 456T; Accession number U85907 (16S rDNA)). The protein is ~90 kDa (similar to nitrate reductase enzymes characterised from alternate bacteria) and stains positive in an in-situ nitrate reduction (native) assay technique. The protein may be N-terminal blocked, although further sequencing experiments are required to confirm this. This work is based upon phenotyped Antarctic bacteria (S. gelidimarina; S.frigidimarina) that was collected during other ASAC projects. (Refer: Psychrophilic Bacteria from Antarctic Sea-ice and Phospholipids of Antarctic sea ice algal communities new sources of PUFA [ASAC_708] and Biodiversity and ecophysiology of Antarctic sea-ice bacteria [ASAC_1012]). The download file contains 4 scientific papers produced from this work - one of these papers also contains a large set of accession numbers for data stored at GenBank. |
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