Sampling of planktonic microorganisms for metagenomic, metaproteomic and metatranscriptomic analyses

No known quality issues. GOS stands for Global Ocean Sampling. TFF- Tangential Flow Filtration. Large volumes of water (200 - 500 L) were filtered and fractionated by size for various planktonic components: eukaryotic phytoplankton, prokaryotic picoplankton, and marine viruses. Sample sites were cho...

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Bibliographic Details
Other Authors: AADC (originator), AU/AADC > Australian Antarctic Data Centre, Australia (resourceProvider)
Format: Dataset
Language:unknown
Published: Australian Ocean Data Network
Subjects:
AMD
IPY
Online Access:https://researchdata.ands.org.au/sampling-planktonic-microorganisms-metatranscriptomic-analyses/685006
https://data.aad.gov.au/metadata/records/CEAMARC_CASO_200708_V3_PLANKTON_GENOMICS_PROTEOMICS
https://data.aad.gov.au/eds/1545/download
https://imicrobe.us/project/view/104
http://www.ncbi.nlm.nih.gov/genbank/
https://secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=2899
http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=CEAMARC_CASO_200708_V3_PLANKTON_GENOMICS_PROTEOMICS
Description
Summary:No known quality issues. GOS stands for Global Ocean Sampling. TFF- Tangential Flow Filtration. Large volumes of water (200 - 500 L) were filtered and fractionated by size for various planktonic components: eukaryotic phytoplankton, prokaryotic picoplankton, and marine viruses. Sample sites were chosen to generate the widest diversity, and included planktonic blooms, oligotrophic zones, small polynyas near sea ice, nearshore areas, and Antarctic bottom water from coastal, canyon and deepwater areas. Half of each sample will be used for DNA library construction, and the other half will be used for meta-proteomic analysis. Random shotgun sequencing of the marine genomic libraries should produce a metagenomic snapshot of planktonic life in a variety of marine habitats. This work was completed as part of ASAC project 2899 (ASAC_2899).