| Summary: | Maintenance and Update Frequency: notPlanned Statement: - Sample collection - The sample sites were selected on the basis of relevance to geographical distribution and temporal variation in the timing of breeding. Three separate sampling events were conducted between 1989 and 2000, and a total of 15 individual colonies were sampled. Five colonies (Beagle Island, North Fisherman Island, Buller Island, Six Mile Island and Kangaroo Island) were sampled on all three occasions. Breeding colonies were visited towards the end of the breeding season, which was calculated by extrapolation from previously published breeding data (Gales et al. 1994). Estimates of pup production for the sampled colonies are taken from Chapter 2 of the thesis. Tissue samples were taken from newly born pups of a single breeding season to ensure the sampling of individuals from their birth colony, and to minimise the sampling of related individuals. Females produce only one pup per season and so each pup represents a single maternal line. Live pups were targeted as the primary source, although freshly dead pups were sampled where available. Exceptions to this occurred on Dangerous Reef and The Pages, where for a number of reasons only dead pups were sampled. Pups were captured by hand, and a small piece of tissue removed from the tip of the middle digit of the right hind flipper or blood was taken from the caudal vein. Tissue was preserved in a solution of 95% EtOH and 100mM EDTA, and blood samples were preserved in liquid nitrogen. For DNA extraction, PCR amplification and DNA analysis see Methods section of Chapter 3 in the thesis. Purpose To use this genetic information to formulate effective policies for the conservation of Australian Sea Lion. Tissue samples were collected from newly born pups from 15 individual colonies between 1989 and 2000, to determine the genetic structure of populations of N. cinerea, with a view to understand the degree of connectedness of different colonies, the role of reproductive strategies in regulating ...
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