Gross Primary Production data collected from the K-Axis voyage of the Aurora Australis, 2016

Progress Code: completed Gross Primary Production Six depths were sampled per CTD station ranging from near-surface to 125 m. Sample depths were based on downward fluorescence profiles and two of six samples always included both near-surface (approximately 5-10 m) and the depth of the chlorophyll ma...

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Bibliographic Details
Format: Dataset
Language:unknown
Published: Australian Ocean Data Network
Subjects:
AMD
Online Access:https://researchdata.edu.au/gross-primary-production-australis-2016/2819565
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Summary:Progress Code: completed Gross Primary Production Six depths were sampled per CTD station ranging from near-surface to 125 m. Sample depths were based on downward fluorescence profiles and two of six samples always included both near-surface (approximately 5-10 m) and the depth of the chlorophyll maximum where applicable. Photosynthetic rates were determined using radioactive NaH14CO3. Incubations were conducted according to the method of Westwood et al. (2011). Cells were incubated for 1 hour at 21 light intensities ranging from 0 to 1200 µmol m-2 s-1 (CT Blue filter centred on 435 nm). Carbon uptake rates were corrected for in situ chlorophyll a (chl a) concentrations (µg L-1) measured using high performance liquid chromatography (HPLC, Wright et al. 2010), and for total dissolved inorganic carbon availability, analysed according to Dickson et al. (2007). Photosynthesis-irradiance (P-I) relationships were then plotted in R and the equation of Platt et al. (1980) used to fit curves to data using robust least squares non-linear regression. Photosynthetic parameters determined included light-saturated photosynthetic rate [Pmax, mg C (mg chl a)-1 h-1], initial slope of the light-limited section of the P-I curve [α, mg C (mg chl a)-1 h-1 (µmol m-2 s-1)-1], light intensity at which carbon-uptake became maximal (calculated as Pmax/ α = Ek, µmol m-2 s-1), intercept of the P-I curve with the carbon uptake axis [c, mg C (mg chl a)-1 h-1] , and the rate of photoinhibition where applicable [β, mg C (mg chl a)-1 h-1 (µmol m-2 s-1)-1]. Gross primary production rates were modelled using R. Depth interval profiles (1 m) of chl a from the surface to 200 m were constructed through the conversion of up-cast fluorometry data measured at each CTD station. For conversions, pooled fluorometry burst data from all sites and depths was linearly regressed against in situ chl a determined using HPLC. Gross daily depth-integrated water-column production was then calculated using chl a depth profiles, photosynthetic parameters (Pmax, α , ...