| Summary: | Progress Code: completed Statement: The unmodified summary description and a fully described list of sampling locations is provided in the word document in the dataset download. This metadata record will contain the results of analyses of tissue samples from Antarctic Rock-cod (Trematomus bernacchii) collected at sites around Davis station to determine wastewater exposure and sub-lethal impact. AAS Project 4177. The results of metal analysis, stable isotope analysis and images of histological analysis of fish from Davis Station are in this dataset. Sample sites and fish collection Antarctic Rock-cod were collected at 6 sites from Prydz Bay near Davis Station East Antarctica, during the 2012/13 summer. Approximately twenty fish were collected from each site by line and in box traps from four sites along a (9 km) spatial gradient starting from the Davis Station wastewater outfall, southward 0km (within 250m of the point of discharge), 1km, 4km and 9km, in the direction of the predominant current. Additionally, two reference sites were sampled 9 km and 16 km north of the discharge point. Once collected, fish were immediately returned to the Davis Station laboratories and sacrificed individually by immersion in an Aqui-s solution (~15ml/L). Once no signs of life were present (approximately 5 min), fish length and weight were measured. Tissues were preserved in various ways for a number of analyses to be conducted at a later date. Stable Isotope analysis. Davis Station Laboratory Dorsolateral muscle tissue from the left side of each individual was removed, placed in aluminium foil and frozen at -20 degrees C for later analysis. Tissue processing A section of frozen tissue was removed (approximately 1 x 1 cm cubed), placed into a clean, acid washed glass crucible and cut into small pieces. This was then dried at 80 degrees C for 48 h. Tissue from each fish was carefully removed and placed into separate 2 ml Eppendorf tubes, each containing an washed, dried stainless steel ball bearing and the lids closed tightly ...
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