| Summary: | Progress Code: completed Statement: Control mortality was lower than ideal and was corrected for using Abbot's correction. Some contamination of hydrocarbon samples occurred during sampling, which test organisms were not exposed to. Contamination levels were therefore excluded from calculations of exposure concentrations. The hydrocarbon content of 0.1% dilutions was unable to be reliably analysed due to accuracy of the equipment and the interfering contamination. Purpose The purpose of the data set was to investigate the response (in terms of abnormality) of larval Sterechinus neumayeri to physically dispersed IFO 180 fuel WAF, IFO 180 chemically dispersed with Slickgone NS and Slickgone NS only treatments. This metadata record contains the results from bioassays conducted to show the response of larval Antarctic Sterechinus neumayeri sea urchins to contamination from combinations if IFO 180 fuel and the fuel dispersant Slickgone NS. AAS project 4142. Experiments used an intermediate grade Fuel Oil (IFO 180) and an internationally approved fuel dispersant, Slickgone NS, produced by Dasic International LTD. Treatments included a physically dispersed treatment of IFO 180 only, a chemically dispersed treatment of IFO 180 treated with Slickgone NS and a Slickgone NS only treatment to determine the toxicity of the dispersant. Treatments were experimentally mixed using a magnetic stirrer to combine treatment substances and filtered seawater (FSW) in temperature-controlled cabinets at 0oC to create a Water Accommodated Fraction (WAF). WAFs were produced in 2 L and 5 L glass aspirator bottles following the methods of Singer, Aurand et al. (2000) with adaptations by Barron and Ka'aihue (2003) and Kostzakoulakis (chemistry section, project 4142) stirring for 42 h with a settling time of 6 h. WAF treatments used concentrations of 100%, 50%, 20% and 10%, CEWAF and dispersant only treatments used concentrations of 10%, 5%, 1% and 0.1%. Toxicity tests were conducted in temperature-controlled cabinets at 0 oC using ...
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