| Summary: | Progress Code: completed Statement: Microsatellite data: Some null alleles were detected, with some individuals not amplifying at individual loci. Fertilisation data: After initial collection prior to sea ice breakout, all urchins collected post sea ice breakout had completed their spawning cycle, preventing repetition of the experiment. Collection dates: Casey Abatus samples: November 13, 2006 to December 14, 2006 Davis Abatus samples: January 27, 2010 to February 25, 2010 Casey Sterechinus samples: January 14, 2009 to January 29 2009 Davis Sterechinus samples: 29 Between November 12, 2009 and February 22, 2010 Population connectivity and gene flow in near shore Antarctic Echinoids (Sterechinus neumayeri, Abatus nimrodi and Abatus ingens) was investigated in East Antarctica. This data set consists of microsatellite genotype data from 11 novel loci and mitochondrial DNA sequences from two gene region, COI and 16S. In addition, to determine if changes in temperature and salinity impacted fertilisation success in S. neumayeri, and to determine the appropriate sperm to egg ratio for this type of experiment, a fertilisation experiment was completed using various combinations of temperature, salinity and sperm to egg ratio. Samples were collected near two Australian Antarctic research stations, Casey and Davis, during the 08/09 and 09/10 summer field seasons. To generate the microsatellite data set, a total of 545 adults, nuemayeri and 26 echinoplutei were collected. Spatial replication was achieved by comparing adult populations between two regions (Casey and Davis). These two regions are separated by approximately 1400 km. Sampling in the Casey region was done at two locations 9 km apart and in the Davis region at five locations separated by 5 - 30 km. Within each location 25-50 individuals were collected from up to three sites approximately 0.5 km apart. Within each site, all individuals were collected within an area less than 50 m2. Adult urchins were collected by dip nets, snorkel or scuba depending on ...
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