Seasonality of phytoplankton growth limitation by iron and manganese in subantarctic waters

• Other Authors: AODN Data Manager (distributor), Antarctic Climate and Ecosystems Cooperative Research Centre (ACE CRC) (hasAssociationWith), Australian Antarctic Program Partnership (AAPP), Institute for Marine and Antarctic Studies, University of Tasmania (hasAssociationWith), Bach, Lennart (author), Bach, Lennart T. (author), Bowie, Andrew (author), Bowie, Andrew R. (author), Bowie, Andrew, Dr (author), Boyd, Philip (author), Boyd, Philip W. (author), Data Officer (distributor), Eggins, Sam (author), Ellwood, Michael (author), Ellwood, Michael J. (author), IMAS Data Manager (hasAssociationWith), Institute for Marine and Antarctic Studies (IMAS) (resourceProvider), Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) (hasAssociationWith), Latour, Pauline (pointOfContact), Latour, Pauline (author), Pier van der Merwe (author), Pinfold, Terry (author), Research School of Earth Sciences (RSES), The Australian National University (ANU) (hasAssociationWith), Strzepek, Robert (author), Strzepek, Robert F. (author), Strzepek, Robert, Dr (author), University of Tasmania (UTAS) (hasAssociationWith), Van Der Merwe, Pier (author), Wuttig, Kathrin (author), van der Merwe, Pier (author)
• Format: Dataset
• Language: unknown
• Published: University of Tasmania, Australia
• Subjects: biota; iron; manganese; co-limitation; radioisotopes; flow cytometry; PHYTOPLANKTON; EARTH SCIENCE; BIOSPHERE; AQUATIC ECOSYSTEMS; PLANKTON; BIOGEOCHEMICAL CYCLES; ECOLOGICAL DYNAMICS; ECOSYSTEM FUNCTIONS; Nitrate; Silicate; Phosphate; Photochemical efficiency of Photosystem II; Functional absorption cross-section of Photosystem II; Dissolved iron concentrations; Dissolved manganese concentrations; Concentration of ammonium {NH4} per unit volume of the water body; Flow cytometry measurements; Global / Oceans | Global / Oceans | Southern Ocean/ Australia extension; Chemical Oceanography; EARTH SCIENCES; OCEANOGRAPHY; Biological Oceanography; Southern Ocean
• Online Access: https://researchdata.edu.au/seasonality-phytoplankton-growth-subantarctic-waters/2759640; https://doi.org/10.25959/BFF2-RC77

Maintenance and Update Frequency: notPlanned Statement: We performed three field bioassays experiments onboard RV Investigator, in the subantarctic zone, to study iron (Fe) and manganese (Mn) co-limitation of phytoplankton growth. The first experiment (spring) was performed at Process Station 2 (45.44°S, 153.31°) during IN2018_V04 and the two other experiments (summer and autumn) were conducted at the Southern Ocean Time Series (SOTS) station (46.80°S, 141.884°E) during IN2019_V02 (autumn) and IN2020_V08 (summer). Unfiltered trace metal clean seawater was collected using a trace metal rosette and incubated with Fe and/or Mn additions in deck-board incubators. Neutral-density mesh bags were used to reproduce the light penetrating the surface ocean. Incubations bottles were sampled at the end of the experiment (day = 7) for macronutrient concentrations, measured through segmented flow analysis, photophysiology, measured using Light-induced Fluorescence Transients Fast Repetition Rate (LIFT-FRR) fluorometer and community composition using flow cytometry. Flow cytometry samples were fixed at sea and analysed back onshore using an Aurora Cytek flow cytometer (Cytek Biosciences). In addition, carbon and iron uptake rates were measured through radioisotopes additions: after 7 days of incubation, a portion of the incubated water was dispensed into smaller bottles and spiked with 14-carbon (NaH14CO3; specific activity 1.85 GBq mmol-1; PerkinElmer, USA) and 55-iron (55FeCl3 in 0.1 M Ultrapure HCl; specific activity 30 MBq mmol-1; PerkinElmer) solutions. Iron and carbon uptake rates were determined by measuring disintegrations per minute (DPM) on a liquid scintillation counter (PerkinElmer Tri-Carb 2910 TR) after incubation of the filters in Ultima Gold liquid scintillation cocktail at least 24h prior to analysis (PerkinElmer). Credit We acknowledge the officers and crew of the RV Investigator (CSIRO Australian Marine National Facility; https://ror.org/01mae9353) for the deployment of all the instruments and the ...